17 S. S. Bibi A. Rehman R. A. Minhas S. Janjua Journal of Animal and Plant Sciences JAPS 20152015/08/01 25 4 1196-1199 1018-7081 NA <p>Five different protocols,<em>&nbsp;viz.&nbsp;</em>Andleeb&nbsp;<em>et al.</em>&nbsp;(2012), Shrivastava&nbsp;<em>et al</em>. (2012), Phenol chloroform DNA extraction method, Roboscreen Kit method and Phenol chloroform triton method, were tested for DNA extraction from skin samples of Leopard (<em>Panthera pardus)&nbsp;</em>preserved in formalin over a period of two to five months. Among these procedures, the Phenol chloroform triton method was proved to be the most efficient protocol with 83% extraction rate and revealed 100% amplification of FCA026 and FCA043 loci. Keeping in views the extraction and amplification efficiency, Phenol chloroform triton method is recommended for molecular studies of formalin preserved skin samples of&nbsp;<em>Panthera pardus</em>.</p> Skin Tissue Samples, DNA Extraction, Polymerase Chain Reaction Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2015-JAPS-161