17 L. Rasool a M. Asghar b A. Jamil c S. U. Rehman Journal of Animal and Plant Sciences JAPS 20152015/08/01 25 4 1081-1086 1018-7081 NA <p>Bacteria play an important role in maintaining the health status of soil ecosystem by preforming many biological processes. Among PGPR,&nbsp;<em>Azospirillum</em>&nbsp;is considered as an important genus which is closely-associated with plants and shows potential to degrade organic contaminants, improve the plant health and increase crops yield.&nbsp;The present study was carried out for the isolation of&nbsp;<em>Azospirillum</em>&nbsp;spp, which can be used as crop inoculants. Four bacterial strains were isolated from wheat rhizosphere. The isolates were characterized on the basis of colony, cell morphology, shape, size and motility.&nbsp;<em>Azospirillum</em>&nbsp;strain Azo LR3 has cream/ pink colony color and plump rod, 3&micro;m in size, with typical helical motility. This isolate showed ability to produce indole-3-acetic acid (11.5 &micro;g/ml of culture medium) and gibberellic acid (12.8 &micro;g/ml of culture medium). 16S rRNA genes sequence analysis revealed that isolate belongs to the genus&nbsp;<em>Azospirillum&nbsp;</em>with 96% similarity and phylogenetic tree represent that it was different from the remaining other member of the genus&nbsp;<em>Azospirillum.</em></p> Azospirillum, rhizosphere, IAA, GA, 16SrRNA Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2015-JAPS-144