17 M. Gohar A. A. Sheikh A. A. Anjum T. Hussain J. Muhammad A. Tabbassum A. Kanwal I. Safdar Journal of Animal and Plant Sciences JAPS 20152015/08/01 25 4 984-988 1018-7081 NA <p>Confirmed<em>&nbsp;E. coli&nbsp;</em>(n = 25) were analyzed for the presence of plasmids and cured using temperature and sodium dodecyl sulphate (SDS) treatments. Plasmid profiling of the isolates showed that 96 % isolates were harboring plasmids of varying size and number. The average plasmid number among the isolates was 2.28, ranging from 0 &ndash; 5 plasmids per isolate and plasmid size varied from 100bp &ndash; 12kb which was distributed randomly in these isolates. Statistically no significant correlation (<em>p</em>&gt;0.05) was found between antibiotic resistance and plasmid profiles. Plasmid curing study showed 3.9% and 16.7% curing efficiency with that of temperature and SDS treatment, respectively.<strong>&nbsp;</strong>Statistical analysis revealed that resistant isolates, treated with either physical or chemical treatments produced significant results (<em>p</em>&lt;0.05) of plasmid curing. Antimicrobial resistance genes might be located on plasmid and statistically no relationship was observed between antibiotic resistance and plasmid profiles.</p> Plasmid profiles, plasmid curing, E. coli, antibiotic resistance Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2015-JAPS-131