17 Soheir Ahmed Al-Masri Journal of Animal and Plant Sciences JAPS 20152015/02/01 25 1 72-77 1018-7081 NA <p>Lead poisoning has been recognized as a major public health risk particularly in developing countries. So the principal aim of this study was occurred to evaluate the protective effect of pumpkin oil and vitamin E on lead induced testicular toxicity in male rats. Forty Sprague Dawley strain rats weighting 165&plusmn;5g were divided into five groups (8 rats each). &nbsp;Groups 1 and 2 acted as negative -ve and positive control +ve, while groups 3, 4 and 5 were administered pumpkin oil, vitamin-E and pumpkin oil plus vitamin E respectively, throughout the study period of the experiment (45 days). After 21 days of the beginning of the experiment, the negative control group -ve was injected with normal saline (1cc) intraperitoneally daily, but control +ve, pumpkin oil and vitamin E groups received lead chloride at a dose 12 mg/kg intraperitoneally daily for twenty four days. Results revealed that lead evoked prominent testicular toxicity, deterioration of sperm characters, and disruption of serum gonad/ pituitary hormone levels luteinizing hormone (LH), follicle stimulating hormone (FSH) and (testosterone). Further, lead markedly raised testicular oxidative-stress (lipid-peroxides), while reduced activity of the antioxidant-enzymes, superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase. These changes were also accompanied by a significant body weight gain along with a rise in food efficiency ratio (FER). Treatment with either or both of vitamin-E and pumpkin-seed oil appreciably ameliorated the Pb-induced anomalies as showed a significantly increased in body weight gain, weigh gain% and FER. Also it showed significant improvement of sperm characters, a significant increase in serum LH, FSH and testosterone hormones and increase in antioxidant enzymes. Combined treatment with both showed a superior testicular protection than their individual responses. These results were agreement with that demonstrated by (Kutlubay&nbsp;<em>et al.,</em> 2006 and Jamilah 2013) who reported that the improvement of reproductive efficiency of male rats by vitamin E is attributed to its powerful antioxidant property. It is concluded that pumpkin oil plus vitamin-E administration could reduce the testicular toxicity of lead in male rats. The adverse effects due to its antioxidant properties</p> Lead, vitamin-E, pumpkin oil, testicular toxicity, rats Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2015-JAPS-11