17 M. S. Ahmed K. S. Ahmed W. A. Khan H. Ali F. Ollevier D. L. Ray Journal of Animal and Plant Sciences JAPS 20142014/02/01 24 1 149-153 1018-7081 NA <p><em>Trypanosoma danilewskyi</em>&nbsp;strain F Cc-1 was isolated from the blood of a laboratory infected juvenile common carp.&nbsp; Trypanosomes (blood stream forms) were first adapted to culture conditions in Tobie&rsquo;s medium.&nbsp; Ten sterilized glass culture tubes each containing 2 ml of Tobie&rsquo;s medium solidified in slants and covered with 1 ml Locke-Krebs overlay, were inoculated with 0.1 ml of infected blood containing 1x 105 live trypanosomes and incubated at 20&ordm; C.&nbsp; Tobie's medium supported growth and trypanosomes were well adapted to culture conditions because small colonies were seen in 6th and 7th subculture.&nbsp; The adapted trypanosomes were re-inoculated in a monophasic liquid culture medium (L4 NHS).&nbsp; Ten sterilized caped glass culture tubes, each containing 3 ml of medium, were inoculated with 0.1 ml culture containing 3 x 105 flagellates and incubated at 20&ordm; C.&nbsp; Trypanosome propagation / multiplication were examined on day 3 p.i. and daily later on and their number ml-1 was estimated by the Rapid Matching Method.&nbsp; Trypanosomes growth obtained in L4 NHS culture medium on day 7-8 was 5.0 &plusmn; 0.56 x 106 live trypanosomes ml-1.&nbsp; The trypanosome number significantly (P&lt;0.05) increased from day 3 to 8 as compared to day 1.&nbsp; Flagellate numbers remained high until day 10 but decreased from day 12-15 p.i.&nbsp; L4 NHS, mono-phasic culture medium supported growth of&nbsp;<em>T. danilewskyi</em> strain FCc-1 (culture forms) significantly high (P&lt;0.01) at 20&ordm; C and found the best for rapid fish trypanosome culture.&nbsp;</p> rapid culture, monophasic medium, strain F Cc-1, L4NHS, blood flagellates, fish Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2014-JAPS-22