17 J. Lee I. Park1and J. Cho Journal of Animal and Plant Sciences JAPS 20142014/08/01 24 4 1116-1122 1018-7081 NA <p>Wheat bran, soybean meal, corn flour and the combinations of these individual substrates with nutritive supplements containing 1% casein hydrolysate, 0.2% CaCl22H2O, 0.4% (NH4)2SO4, 0.02% MgSO47H2O, 0.05% KH2PO4 and0.04% K2HPO4 were evaluated to select an optimal medium in solid state fermentation (SSF) to produce extracellular phytase from a bacterial strain,&nbsp;<em>Bacillus</em> sp. T4. The combination of corn flour with nutritive supplements resulted in best phytase synthesis and was used for further SSF explorations. Maximum phytase production (20787&plusmn;39 U/g) was observed at a growth period of 84 h, 55.5% moisture content, and 4% inoculum density. Optimum pH for phytase production was 7.0. Enzyme activity was enhanced (P&lt;0.05, 16496&plusmn;187 to 18304&plusmn;187 U/g) in the presence of glucose and galactose as a carbon source. The additional nitrogen sources impaired (P&lt;0.05) the phytase activity, and corn steep liquor and sodium nitrateseverely inhibited the enzyme synthesis. T4 phytase produced in SSF may be a promising strategy for upgrading the nutritional quality and combating environment pollution in the feed industry.</p> Solid state fermentation, phytase, Bacillus sp., feed industry Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2014-JAPS-161