17 A. Hussain S. U. Rehman S. Aslam N. Javed Z. Abbas Journal of Animal and Plant Sciences JAPS 20142014/08/01 24 4 1110-1115 1018-7081 NA <p>In this study, an immunoassay is developed for qualitative determination of NS1 antigen of dengue virus in serum using nano-gold particles. This diagnostic device comprises of four components such as sample pad, conjugation pad, nitrocellulose membrane and absorbent pad. Anti-NS1 antibodies and rabbit anti-mouse IgG antibodies were coated on nitrocellulose membrane as a test and control line respectively. Assay device was evaluated by purified NS1 and prior ELISA confirmed NS1 positive and negative samples. The diagnostic device showed promising results to detect NS1.</p> Dengue Virus, Diagnostic Device, Colloidal Gold Particles, Rapid Diagnosis, NS1 antigen, ELISA Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2014-JAPS-160