17 M. Karatas M. Aasim M. Çiftçioğlu Journal of Animal and Plant Sciences JAPS 20142014/06/01 24 3 838-842 1018-7081 NA <p>Roundleaf toothcup<strong><em>&nbsp;-&nbsp;</em></strong><em>Rotala rotundifolia&nbsp;</em>[(Buch-Ham. ex Roxb) Koehne] is an important aquatic, ornamental and medicinal plant of family Lythraceae. Present study was designed to propagate adventitious shoots regeneration from leaf explants cultured on MS medium supplemented with 5 levels of &nbsp;0.25-2.00 mg/L BA. Maximum shoot regenration frequency (53.33 percent) and mean number of 17.06 shoots per explant was reocrded on MS medium supplemented with 1.0 mg/L BA. Multiple shoots buds were also induced on all culture mediums most of which were either slow to grow or failed&nbsp; to convert in to shoots. Transfer of explants to 0.20 mg/L GA3 propmoted the shoot regeneration frequecy to 100 percent on MS medium containing 1.00 mg/L BA and increased mean number of shoots (24.0) on MS medium supplemented with 0.25 mg/L BA-0.20 mg/L GA3. The developing shoots rooted in the shoot elongation medium showed positive effects of BA- 0.20 mg/L GA3 combinations. Thereafter, the developing plantlets were acclimatised in water using pH level of 4.0-10 (7 levels). All plants at pH 4.0 developed necrosis and died; whereas, rest of the plants showed variable grpwth at other pH levels. A comparison among the pH treatments suggested slightly acidic to alkaline medium (pH 6-9) was more appropriate for the acclimatisation of&nbsp;<em>in vitro</em> regenerated plants without having negative effects on plant growth and development.</p> Acclimatisation, Adventitous, Aquatic, In vitro, Shoots, Regeneration Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2014-JAPS-118