17 Q. Shahzad A. Riaza T. Akhter A. Sattara J. Nazirb A. A. K. Niazi A. Azamc A. U. Husnac Journal of Animal and Plant Sciences JAPS 20142014/06/01 24 3 765-769 1018-7081 NA <p>Small ovarian size and low number of follicles are major causes of low reproductive potential in buffalo (<em>Bubalus</em>&nbsp;<em>bubalis</em>). There is no tool through which reproductive performance of buffalo can be phenotyped. Therefore, aim of the present study was to develop a method on the basis of which reproductive performance of buffaloes can be phenotypically assessed. For this purpose two experiments were designed. In first experiment, ten Nili-Ravi buffalo heifers were used in the study to measure repeatability of follicular count. Each heifer was scanned on alternate day, from day 1 to day 9 of the successive estrous cycle. Antral follicles and repeatability was assessed in different follicular waves. In 2nd experiment, 4 lactating buffaloes were selected to count antral follicles from day 1 to day 10 of the estrous cycle and super-stimulated by using FSH hormone. Follicular count after FSH injection was examined by ultrasound. Follicular count is highly repeatable in different follicular waves within (r = 0.83) and between (r = 0.85) different estrous cycles. Follicular count and super stimulatory response are positively correlated (r = 0.95). In conclusion, animals with high antral follicular count can be selected for super stimulation and embryo transfer to get optimum response.</p> Antral follicular count, Super-stimulation, Repeatability, Buffalo Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2014-JAPS-108