17 M. T. Azhar Z. I. Anjum S. Mansoor Journal of Animal and Plant Sciences JAPS 20132013/10/01 23 5 1436-1440 1018-7081 NA <p>The diploid cotton species belonging to A, D and tetraploids with AD genome were screened for the presence/absence of components of cotton leaf curl complex. These cotton species are being maintained in a living herbarium for more than fourdecades under natural conditions. As expected, the two diploid species (<em>Gossypium arboreum</em>&nbsp;and&nbsp;<em>G. herbaceum</em>) of A genome, were found to be free of virus when screened by PCR and &Phi;29 DNA polymerase. The two cotton species of D genome (<em>G. thurberii</em>&nbsp;and&nbsp;<em>G. aridum</em>) showed the presence of begomovirus. The species belonging to AD genome were found to be susceptible to CLCuD due to the presence of both begomovirus and betasatellite. However, the interesting exception among D genome species was&nbsp;<em>G. gossypioides</em>. The results from present study suggest that<em>&nbsp;G. gossypioides</em>&nbsp;is a valuable resource for mapping of resistance by developing segregating populations and for developing synthetic tetraploids.</p> Resistance, susceptibility, Cotton leaf curl disease, betasatellite Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2013-JAPS-534