17 Chunmei Guo Shuqing Liu Tihua Zheng Ming-Zhong Sun Journal of Animal and Plant Sciences JAPS 20132013/10/01 23 5 1398-1406 1018-7081 NA <p>The snake of&nbsp;<em>Gloydius shedaoensis shedaoensis</em>&nbsp;(GSS) is the particular and sole snake species from Shedao island in Dalian, China. A novel gene named as GSSG-TLE was cloned from GSSG by PCR using GSSG-cDNA library plasmid as template. The GSSG-TLE cDNA was sequenced and encompasses an open reading frame of 786 bp in length and encoding a protein of 262 amino acid residues. The&nbsp;<a id="OLE_LINK46" name="OLE_LINK46">GSSG-TLE</a>&nbsp;gene has a base composition of 14 Ala, 12 Cys, 14 Asp, 13 Glu, 10 Phe, 19 Gly, 7 His, 20 Ile, 15 Lys, 25 Leu, 4 Met, 12 Asn, 19 Pro, 7 Gln, 12 Arg, 20 Ser, 14 Thr, 13 Val, 5 Trp and 7 Tyr. GSSG-TLE includes a signal peptide of 18 amino acids, a proposed propeptide of 6 amino acids and a matured peptide of 238 amino acids. It also contains 12 Cys which form 6 disulfide bridges and has three conserved catalytically active sites of His67, Asp112 and Ser202. Sequence comparison revealed that GSSG-TLE amino acids sequence shared highly identity with other snake venom<a id="OLE_LINK45" name="OLE_LINK45">&nbsp;thrombin-like enzyme</a>s.</p> Gloydius shedaoensis shedaoensis, Snake, Thrombin-like enzyme Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2013-JAPS-529