17 Q. Akram K. Muhammad M. Rabbani J. Nazir M. Nawaz K. Hanif Z. Shakoor Journal of Animal and Plant Sciences JAPS 20132013/06/01 23 3 771-774 1018-7081 NA <p>Foot and Mouth Disease (FMD) virus grew well on BHK-21 cell line. The virus showed poor tissue culture infective dose 50 (TCID50) that was log 103.2&plusmn;0.2 in BHK-21 cell line having Glasgow Minimal Essential Medium (GMEM) without Fetal Calf Serum (FCS). Addition of one per cent FCS in the medium @ one percent increased log 107.1&plusmn;0.2 units of virus TCID50. Incubation temperature of 350 C and 370 C supported the multiplication and maintenance of BHK-21 cells and yielded log106.6&plusmn;0.1 and log107.0&plusmn;0.2units of virus TCID50, respectively. Each serotype of FMD virus showed log106.29&plusmn;0.07 units of virus TCID50 in the stationary monolayer of BHK-21 cells in roux flask (75cm 2), log107.66&plusmn; 0.02 units of virus TCID50 in roller bottles (490cm2) and log108.34 &plusmn; 0.07 units of virus TCID50 on 0.2 g of micro-carriers suspending in 200 ml of the growth medium in stirring bottle. The infectivity titer/TCID50 of each of the virus serotypes was significantly higher in roller bottles than that achieved in roux flasks (p&lt;0.05) and was significantly higher in stirring bottle containing micro-carriers suspending in the growth medium than that of harvested in roller bottle (p&lt;0.05). It is concluded that the infectivity titer of the virus is directly proportional to number of BHK-21 cells in the culture system.</p> Anchorage dependant BHK-21 cells, roller bottles, infectivity titer, FMD virus Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2013-JAPS-315