17 Q. Khan Z. U. Rehman M. U. Samo A. Raziq I. Ahmad F. A. Khan M. Younus F. H. Samo D. Muhammad Journal of Animal and Plant Sciences JAPS 20122012/04/01 22 2 51-54 1018-7081 NA <p>his study was designed to determine conception rate in Kundhi buffaloes treated with prostaglandin F2 alpha (PGF2&alpha;) after timed insemination and simultaneous treatment with or without gonadotrophin releasing hormone (GnRH). A total 40 anestrus Kundhi buffaloes were randomly selected in the vicinity of Sindh Agricultural University, Tandojam and were divided in to 4 equal groups, each comprising of ten animals (n=10). Animals in group A, were injected intramuscularly with 2 ml Dalmazine, a synthetic analogue of prostaglandin PGF2&alpha; (2 ml Dalmazine = 0.150 mg cloprostenol, Fatro, Itly), on day 1 followed by the same treatment on day 11 with fixed time artificial insemination (72 h) after the 2nd dose of prostaglandin injection. In group B, same treatment as in group A but in addition gonadotrophin releasing hormone (GnRH) @2 ml of Dalmarelin (Lecirelin aetate Fatro, Itly), a synthetic analogue of GnRH was injected at the time of fixed time AI. In group C, same protocol as in group A, but with 2 ml of Dalmazine and 2 ml of Dalmarelin were injected simultaneously on day 11. In group D, animals were kept as control. The conception rate in group A, B, C and D was 20.2%, 30.3%, 30.3% and 10.1% respectively. The mean conception rate in treated group was higher (26.6%) than control (10.1%). It was concluded from the present study that a 16.6% increase in conception rate was achieved in anoestrus Kundhi buffalo through hormonal therapy in field condition.</p> Prostaglandin, Kundhi buffalo, Insemination, GnRH, Conception rate Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2012-JAPS-2010