17 N. Rashid M. A. Bajwa M. Rafeeq M. A. Khan Z. Ahmad M. M. Tariq A. Wadood F. Abbas Journal of Animal and Plant Sciences JAPS 20122012/02/01 22 1 NA 1018-7081 NA <p>Aflatoxin contamination of poultry feed results in heavy economic loss and a potential risk for human being. Finished commercial broiler feed (n=96) samples collected from different broiler farms for the period June 2009 to May 2010 from south west Pakistan were analyzed for aflatoxin B1(AfB1) by using thin layer chromatography with a detection limit of 3&micro;g/kg. Analysis revealed that 91.66% samples were positive with a range 10 to 166&micro;g/kg and mean 47.64&plusmn;2.55&micro;g/kg. Highest level of AfB1 was found in the month of September ranged from 42 to 166&micro;g/kg with mean of 83.25&plusmn;16.11&micro;g/kg. Categorically 82.30% samples were above the permissible limit recommenced by United States Food and Drug Administration. A significant (P&lt;0.05) effect of season on aflatoxin production was noted. The prevalence of AfB1 may be attributed to poor harvesting technique, insect infestation, storage or environmental conditions.</p> aflatoxin, broiler, poultry feed, prevalence. Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2012-JAPS-140