17 A. W. Yunus J. Böhm Journal of Animal and Plant Sciences JAPS 20112011/04/01 21 2 303-304 1018-7081 NA <p>A simplification and standardisation of the existing methods to produce aflatoxin B1 (AFB1) on rice medium is described. As per conventional method, rice were first soaked in 1 ml water/2 g of rice for 2 hr, autoclaved, and then inoculated with&nbsp;<em>Aspergillus flavus</em>. During different stages of incubation, water was added according to different regimens. It was concluded that 1 ml water/100 g of rice at 24 to 36 hr of incubation is the maximum amount of water that could be added for optimal AFB1 production.</p> Aflatoxin, Rice, Aspergillus flavus Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2011-JAPS-215