17 F. Abbas F. A. Khan A. Hussain F. Ahmed M. Ahmed S. Ahmed M. Z. Ahmed M. A. Attique A. Wadood M. K. Taj. Journal of Animal and Plant Sciences JAPS 20102010/08/01 20 4 315-317 1018-7081 NA <p>Kid kidney cells form less than 2 months of age were grown, 80% confluent monolayers were obtained after 10-12 days.&nbsp; &nbsp;Live attenuated goat pox virus vaccine (Gorgan 55, Iranian) was used to infect the confluent monolayers.&nbsp; &nbsp;Eighty percent cytopethic effect (CPE) was obtained on day 5 of virus inoculation.&nbsp; TCID50 calculated for the pool virus was 10 5.5/ ml.&nbsp; Vaccine was found free from any bacterial and mycoplasma contamination. All mice and guinea pigs were found healthy after 21days post vaccinal administration.&nbsp; Vaccinated goats challenged after 14 days with 1 ml of virulent goat pox virus (TCID50 104.5/ml) did not show any unwanted reaction while controls challenged with 1 ml of virulent goat pox virus (TCID50 10 3.5/ml)&nbsp; showed pox lesions and rise in body temperature. The main objective of this study was to produce and study the efficacy of this vaccine.</p> attenuated, goat pox virus, confluent, monolayer, virulent, challenge Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2010-JAPS-411