17 S. Rahman A. Ahmad S. Bhatti Journal of Animal and Plant Sciences JAPS 20062006/04/01 16 2 NA 1018-7081 NA <p>A study was conducted to evaluate the growth promoting effect of FCS and its proper dilution in cell culture media for persistent cultivation and maintenance of BHK-21 cell culture, by comparing it with other animal sera. Different animal sera of different species as cattle calf serum , Allanto-amniotic fluid , Sheep serum , Poultry serum and Goat serum @ 10% were added to cell culture medium (M 199, Biomedicals; USA). A fast and reliable technique, MTT (3 {4, 5-Dimethyzol-2-Yl} 2, 5-Diphenyl Tetrazolium Bromide) Assay was employed to measure the cell densities in small amount of cell culture media by measuring OD values under 570nm, with 630nm as a reference read out. It was concluded that FCS is the best growth promoting agent for BHK-21 cell line and 5-7.5 % concentration has the desired effect of persistent cultivation and maintenance of BHK-21 cell line.</p> BHK-21 cells, fetal calf serum, cell density, growth promoting factor Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2006-JAPS-215