[{
  "type": "article-journal",
  "title": "EFFECTS OF DIFFERENT DOSES OF SHIITAKE MUSHROOM (LENTINUS EDODES) ON SPERM PARAMETERS AND TESTICULAR TISSUE IN RATS: AN EXPERIMENTAL STUDY",
  "author": [
    {
      "family": "Büyük",
      "given": ""
    },
    {
      "family": "Demir",
      "given": ""
    },
    {
      "family": "Eroğlu",
      "given": ""
    },
    {
      "family": "Öztopuz",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2023]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "33",
  "issue": "2",
  "page": "389-396",
  "DOI": "https://doi.org/10.36899/JAPS.2023.2.0629",
  "abstract": "<p><em>Lentinus edodes</em>&nbsp;has been cultivated and used as food and medicine in far eastern countries.The present study aimed to reveal whether&nbsp;<em>L.edodes</em>&nbsp;that is used for different purposes has a toxic effect on the male genital system and to investigate its relationship with male infertility by showing its effects on sperm parameters. Twenty-four male Wistar rats were randomly divided into three groups as Control (C), Low-dose (LD) and High-dose (HD). Rats in Control were administered saline physiological once a day for 7 days. The LD and HD groups received 100 and 400mg/kg of&nbsp;<em>L.edodes</em>&nbsp;extract, respectively, once daily for 7 days. At the end of 7 days of administration of treatments, semen analysis, micronucleus analysis, gene expression levels and testicular Johnsen scoring was performed. Comparisons between groups without normal distribution were analyzed using Kruskal&ndash;Wallis variance analysis and those between groups with normal distribution were analyzed using one-way analysis of variance, followed by Tukey&rsquo;s test. P values of &lt;0.05 were considered statistically significant. It is demonstrated that both low and high doses of extract significantly reduced spermatogenesis in seminiferous tubules found in the testicles of rats. In addition, high-dose administration resulted in decreased total sperm counts, whereas the progressive and non-progressive sperm motility counts were significantly decreased in both experimental groups. The gene expression levels of Casp-3 and TNF-&alpha; were increased in both the LD and HD groups compared with that in the C. Oral administration of low and high doses of&nbsp;<em>L.edodes</em> led to reduced spermatogenesis in the testicles, tissue damage to the testicle and decreased motile and total sperm counts.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=VS-21-0029"
}]