[{
  "type": "article-journal",
  "title": "ANTIBACTERIAL ACTIVITY OF TRIFLUOPERAZINE; IN VITRO SUSCEPTIBILITY OF MRSA STAPHYLOCOCCUS AUREUS, PSEUDOMONAS AERUGINOSA AND E. COLI, AND IN VIVO EVALUATION AGAINST METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS IN SURGICAL WOUND INFECTION MODEL",
  "author": [
    {
      "family": "Ashok1",
      "given": ""
    },
    {
      "family": "Ejaz",
      "given": ""
    },
    {
      "family": "Manzoor",
      "given": ""
    },
    {
      "family": "Qadir2",
      "given": ""
    },
    {
      "family": "Nazir",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2021]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "31",
  "issue": "5",
  "page": "1287-1292",
  "DOI": "https://doi.org/10.36899/JAPS.2021.5.0329",
  "abstract": "<p>Management of surgical site infections (SSI) becomes very difficult due to Bacterial resistance. Current study was designed to determine&nbsp;<em>in vitro</em>&nbsp;susceptibility and minimum inhibitory concentration of trifluoperazine against MRSA,&nbsp;<em>Staphylococcus aureus</em>,&nbsp;<em>Pseudomonas aeruginosa</em>&nbsp;and&nbsp;<em>E. coli</em>, and determination of&nbsp;<em>in vivo</em>&nbsp;antibacterial activity of trifluoperazine in induced surgical site infections by MRSA.&nbsp;<em>In vitro</em>&nbsp;antibacterial activity of trifluoperazine was determined by disc diffusion susceptibility testing while determination of MIC by agar dilution technique.&nbsp;<em>In vivo</em>&nbsp;antibacterial activity was observed by microbiological assessment of tissue harvested from the experimentally infected wound site and number of colony forming units per gram (cfu/gm) of tissue. The zones of growth inhibition of trifluoperazine were determined against&nbsp;<em>MRSA, E. coli</em>&nbsp;and&nbsp;<em>Pseudomonas aeruginosa.&nbsp;</em>At a disc potency of 25 &micro;g no zone of inhibition was produced against any isolate. 50 &micro;g produced 13 mm and 16 mm zone of inhibition against&nbsp;<em>MRSA and Pseudomonas aeruginosa&nbsp;</em>respectively. Whereas, No zone against E. coli was observed at this concentration but, 12mm, 15mm and 18mm zones of inhibition were produced by 100<em>&nbsp;&micro;g,&nbsp;</em>150 &micro;g<em>,&nbsp;</em>and 200<em>&nbsp;&micro;g</em> per disc concentration of trifluoperazine respectively. The colony forming units/gram observed in trifluoperazine treated wounds were 2.93&plusmn;0.02 x 10⁶ while in case of normal saline treated wounds the value was 6.68&plusmn;0.07 x 10⁶. Results revealed the efficacy of trifluoperazine as a potentially therapeutic agent in the treatment of wound infection beds especially against MRSA.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=VS-20-0008"
}]