[{
  "type": "article-journal",
  "title": "PHYTOCHEMICAL ANALYSIS AND IN VITRO INVESTIGATION OF ANTI-INFLAMMATORY AND XANTHINE OXIDASE INHIBITION POTENTIAL OF ROOT EXTRACTS OF BRYOPHYLLUM PINNATUM",
  "author": [
    {
      "family": "Latif1",
      "given": ""
    },
    {
      "family": "Ashiq",
      "given": ""
    },
    {
      "family": "Ashiq",
      "given": ""
    },
    {
      "family": "Ali",
      "given": ""
    },
    {
      "family": "Qamar",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2020]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "30",
  "issue": "1",
  "page": "219-228",
  "DOI": "https://doi.org/10.36899/JAPS.2020.1.0025",
  "abstract": "<p>The aim of present study is to emphasize the phytochemical and biological studies of root extracts of&nbsp;<em>Bryophyllum pinnatum</em>, as little work was documented on the roots. Phytochemical analysis of powder and extracts were evaluated by the standard methods. UV/ Visible scanning, FTIR and atomic absorption spectroscopy were also performed for the purposes of standardization.&nbsp;<em>In vitro</em>&nbsp;anti-inflammatory investigation was done by protein denaturation method and xanthine oxidase inhibition assay was performed to confirm anti-gout effect. Phytochemical analysis results revealed the presence of primary metabolites (%w/w); carbohydrates (48.54<u>+</u>0.6) &gt; proteins (21.06<u>+</u>0.6) &gt; lipids (1.32<u>+</u>0.001). Secondary metabolites (mg/g) were also isolated; methanol extract has higher concentrations of flavonoids (95.81<u>+</u>0.026) and glycosaponins (39.9<u>+</u>0.103) while in water extract polysaccharide (139.4&nbsp;<u>+</u>&nbsp;0.026) and polyphenols (114.93<u>+</u>0.0234) were found to be in maximum quantities as compared to the other extracts. UV/Visible and FTIR scanning results have shown the presence of various significant functional groups.&nbsp;<em>In vitro</em>&nbsp;anti-inflammatory evaluation showed that aqueous extract (87.46%) was found to be the most active with IC50 (570.24ug/ml) as compared to the reference drug diclofenac sodium.&nbsp;<em>In vitro</em> xanthine oxidase inhibition activity was performed and methanol extract (88.24%) found to be the most effective. The findings confirm the plant nutritional value and rationale behind its use in traditional medicine for the treatment of inflammation and gout.&nbsp;</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=Pharm-18-0018"
}]