[{
  "type": "article-journal",
  "title": "GC/MS PROFILING, ANTIBACTERIAL AND ATOMIC FORCE MICROSCOPIC STUDY OF BACTERIAL CELL MEMBRANE AFFECTED BY FARSETIA HELIOPHILA BARK EXTRACT ALONG WITH WOUND HEALING ACTIVITY",
  "author": [
    {
      "family": "Burki",
      "given": ""
    },
    {
      "family": "Burki",
      "given": ""
    },
    {
      "family": "Mehjabeen",
      "given": ""
    },
    {
      "family": "Khan",
      "given": ""
    },
    {
      "family": "Ahmed",
      "given": ""
    },
    {
      "family": "Ali",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2022]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "32",
  "issue": "3",
  "page": "877-885",
  "DOI": "http://doi.org/10.36899/JAPS.2022.3.0489",
  "abstract": "<p>The aim of the study was to evaluate phytochemically, biologically and pharmacologically&nbsp;<em>Farsetia heliophila&nbsp;</em>(<em>F. heliophila</em>) using&nbsp;<em>in vivo</em>&nbsp;wound healing technique. Phytochemically,&nbsp;<em>F. heliophila</em>&nbsp;positive test for tannins, saponins, flavonoides, triterpenoids and alkaloids. The chemical composition of&nbsp;<em>F. heliophila</em>&nbsp;evaluated through gas chromatography (GC), gas chromatography/mass spectroscopy (GC/MS) and Fourier transformed infra-red (FTIR) reveled that&nbsp;<em>F. heliophila</em>&nbsp;contain important bioactive compounds like barrigenol R1, ethyl iso-allocholate, lupeol acetate, isorhamnetin, &alpha;-amyrine, &alpha;-tocopherol, l-(+)-ascorbic acid 2,6-dihexadecanoate, ascorbyl palmitate, 2-pyrazolin-5-one, 3,4,4-trimethyl- isoquercetin, pyrimidine, indole and cyclolaudenol. The crude extract was further evaluated for antibacterial activity, (AFM) study of extract treated bacterial cells, acute oral toxicity and&nbsp;<em>in vivo</em>&nbsp;wound healing potential. The MIC<sub>50&nbsp;</sub>values of&nbsp;<em>F. heliophila&nbsp;</em>extract against&nbsp;<em>B. subitils</em>,&nbsp;<em>S. typhi&nbsp;</em><em>E. coli&nbsp;</em>and&nbsp;<em>P. aeruginosa</em>&nbsp;were 25, 50, 75 and 100 &micro;g/mL at (p&lt;0.01) and (p&lt;0.05). The AFM images showed that the cell membrane of&nbsp;<em>B. subitils</em>,&nbsp;<em>S. typhi</em>&nbsp;and&nbsp;<em>E. coli&nbsp;</em>were significantly damaged with cytoplasm leaked from the bacterial cell.&nbsp;<em>P. aeruginosa</em>&nbsp;cell membrane was partially damaged. The extract did not show any acute toxicity at higher dose of 2000, 3000 and 5000 mg/kg. The results of wound healing capabilities showed that the wounds were significantly healed in animals treated with&nbsp;<em>F. heliophila&nbsp;</em>extract at 10 and 15 % ointment dose at (<span lang=\"SR-LATN-RS\">p&lt;0.01 and p&lt;0.001). The&nbsp;</span>epithelialization process was also accelerated at 10 an 15 % dose of&nbsp;<em>F. heliophila&nbsp;</em>extract ointment and took&nbsp;<span lang=\"EN-AU\">20.4&plusmn;2.0 and</span><span lang=\"EN-AU\">18.5&plusmn;1.2</span>&nbsp;days for complete wound healing. The results of this study provide scientific support for alternative use of&nbsp;<em>F. heliophila&nbsp;</em>as a therapeutic agent in the treatment of skin wounds and infections.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=Pharm 20-0010"
}]