[{
  "type": "article-journal",
  "title": "ANTI-OXIDATIVE EFFECT OF L-LYSINE ON POST THAW QUALITY OF NILI-RAVI BUFFALO BULL SEMEN (BUBALUS BUBALIS)",
  "author": [
    {
      "family": "Ahmed",
      "given": ""
    },
    {
      "family": "Ayaz",
      "given": ""
    },
    {
      "family": "Mughal",
      "given": ""
    },
    {
      "family": "Rehman",
      "given": ""
    },
    {
      "family": "Mushtaq",
      "given": ""
    },
    {
      "family": "Yousaf",
      "given": ""
    },
    {
      "family": "Din",
      "given": ""
    },
    {
      "family": "Riaz",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2020]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "30",
  "issue": "3",
  "page": "612-618",
  "DOI": "https://doi.org/10.36899/JAPS.2020.3.0072",
  "abstract": "<p>This study was designed to investigate the effect of L-lysine on post-thaw quality of Nili-Ravi buffalo bull semen. Twenty eight ejaculates were collected from four Nili-Ravi buffalo bulls. Semen samples were pooled and split into five equal aliquots and diluted in Tris-citric acid extender containing different concentrations of L-lysine (0.25, 0.5, 1 and 2mM). As a control, semen was extended without addition of L-lysine. The semen samples were cryo-preserved in liquid nitrogen. Post-thaw semen assessment revealed that sperm motility, viability, plasma membrane integrity, acrosomal integrity, mitochondrial integrity and DNA integrity were significantly improved (P &le; 0.05) in 2mM L-lysine supplemented group as compared to control. For other groups supplemented with L-lysine; post-thaw motility, acrosomal integrity and mitochondrial integrity were also improved (P &le; 0.05) for 1mM treated group as compared to control. DNA integrity was improved significantly by all L-lysine treated groups as compared to control. Malondialdehyde concentration was higher (P &le; 0.05) in control group as compared to L-lysine treated groups. Significantly higher rate of catalase was recorded for 1mM and 2mM L-lysine treated groups as compared to control. In conclusion, the supplementation of 2mM L-lysine in semen extender can be used for cryopreservation of Nili-Ravi buffalo bull semen.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=IBC-19-044"
}]