[{
  "type": "article-journal",
  "title": "COMPARISON OF PHYTOCHEMICALS AND ANTIOXIDANT CAPACITY OF HYPERICUM PERFORATUM; WILD PLANT PARTS AND IN VITRO SAMPLES",
  "author": [
    {
      "family": "1",
      "given": ""
    },
    {
      "family": "Önlü",
      "given": ""
    },
    {
      "family": "Ahmed",
      "given": ""
    },
    {
      "family": "Erenler",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2022]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "32",
  "issue": "2",
  "page": "596-603",
  "DOI": "http://doi.org/10.36899/JAPS.2022.2.0459",
  "abstract": "<p>The aim of study is to compare the phytochemicals and free radical scavenging activities in methanol extracts of flower, leaf, stem&nbsp;<em>in vitro&nbsp;</em>plantlet, callus of&nbsp;<em>Hypericum perforatum</em>&nbsp;L.&nbsp;<em>In vitro&nbsp;</em>cultures of<em>&nbsp;H. perforatum&nbsp;</em>was established by using MS-B5 medium contained plant growth regulators such as BAP, TDZ and picloram. Total phenolics and flavonoids were analysed by spectrophotometric methods.The stem was the richest in total phenolics (228.9 mg GAE/g extract) and flavonoids (102.4 mg QE/g extract). Quinic acid, gallic acid, (+)-catechin, ferulic acid, vanillic acid,&nbsp;<em>p</em>-coumaric acid, caffeic acid, and quercetin were determinated by LC-MS/MS. Free radical scavenging activities (ABTS and DPPH) of all samples were detected as IC<sub>50</sub>&nbsp;values, and was compared to standards such as trolox and ascorbic acid. As a result, the stem exhibited the stronger antioxidant activities than other samples, and vanillic acid, ferulic acid and gallic acid could be produced by&nbsp;<em>in vitro</em> culture.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=Biot-20-0026"
}]