[{
  "type": "article-journal",
  "title": "EVALUATION OF ANTIMICROBIAL, ANTIOXIDANT AND ENZYME INHIBITION ROLES OF POLAR AND NON-POLAR EXTRACTS OF CLITORIA TERNATEA SEEDS",
  "author": [
    {
      "family": "Mushtaq1",
      "given": ""
    },
    {
      "family": "Khan",
      "given": ""
    },
    {
      "family": "Seher",
      "given": ""
    },
    {
      "family": "Shahid",
      "given": ""
    },
    {
      "family": "Shahzad",
      "given": ""
    },
    {
      "family": "Bhatti",
      "given": ""
    },
    {
      "family": "Sikander",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2026]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "31",
  "issue": "5",
  "page": "1405-1418",
  "DOI": "https://doi.org/10.36899/JAPS.2021.5.0342",
  "abstract": "<p><em>Clitoria ternatea</em>&nbsp;is one of the medicinal plants of fabaceae family that have been traditionally useful in herbal medicine. In present study, the&nbsp;<em>invitro</em>&nbsp;antimicrobial, antioxidant and enzyme inhibition assay of polar and non-polar extracts/fractions of&nbsp;<em>Clitoria ternatea&nbsp;</em>seeds were evaluated. The antimicrobial activity was done by well diffusion assay while MIC was done by serial dilution method. Crude ethanolic extract showed maximum antibacterial activity against&nbsp;<em>B. subtilus</em>&nbsp;(17.6 mm &plusmn;0.17). Ethyl acetate extract showed maximum MIC at&nbsp;<span lang=\"EN-GB\">0.156 &mu;g/mL against&nbsp;<em>E. coli</em>.</span>&nbsp;In addition, Crude methanolic extract showed maximum antifungal activity against&nbsp;<em>Aspergillus parasiticus</em>&nbsp;(<span lang=\"EN-GB\">16 mm &plusmn;0.27)</span>. The antioxidant activity was done by DPPH and FRAP assay. Crude methanolic extract showed maximum antioxidant activity by DPPH assay (86.26%) while crude methanolic extract exhibit maximum reducing power&nbsp;<span lang=\"EN-GB\">370.19 % &plusmn; 20.50</span>. Moreover,&nbsp;<em>n-</em>hexane extract possessed highest phenolic content (272.16 &plusmn; 17.96 GAE &micro;g/g), crude methanolic extract possessed highest flavonoid content (275.5 &plusmn; 8.24 GAE &micro;g/g). The highest enzyme inhibition assay was observed in crude methanolic extract (&alpha;-amylase),&nbsp;<em>n-</em>hexane extract and water-soluble residue exhibited 50% pepsin enzyme inhibition. The results concluded that most of the extracts/fractions obtained from&nbsp;<em>Clitoria&nbsp;</em>seeds showed significant biological activities and can act as promising source of natural therapeutic agents and can be used for future drug developmental studies.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=Biot-19-0002"
}]