[{
  "type": "article-journal",
  "title": "EFFECT OF GELLING MATRIX COMPOSITION, STORAGE CONDITIONS AND CAPSULE BREAKAGE ON GERMINATION OF ROSA INDICA SYNTHETIC SEEDS",
  "author": [
    {
      "family": "Gull",
      "given": ""
    },
    {
      "family": "Noreen",
      "given": ""
    },
    {
      "family": "Aslam",
      "given": ""
    },
    {
      "family": "Abbas1",
      "given": ""
    },
    {
      "family": "Athar",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2019]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "29",
  "issue": "1",
  "page": "109-116",
  "DOI": "N/A",
  "abstract": "<p>Storage of germplasm using synthetic seed technology is useful to avoid somaclonal variations as well as to cut down the cost and labor of germplasm maintenance under&nbsp;<em>in vitro</em>&nbsp;culturing conditions. In the present study, different parameters that affect the viability and regrowth ability of&nbsp;<em>Rosa indica&nbsp;</em>germplasm stored as synthetic seeds were optimized. The compact, firm, transparent synthetic seeds showing maximum regeneration rate (100 %) were prepared and stored in three different types of air tight storage vessels (glass jars, polypropylene tubes and plastic bags) at 4 &deg;C and 25 &deg;C for various time durations (0-8 weeks). No significant difference in regeneration frequency of&nbsp;<em>Rosa indica&nbsp;</em>synthetic seeds was recorded at different storage conditions. However, the storage temperature significantly influenced the viability and regrowth ability of synthetic seeds. The survival rate (80%) of encapsulated explants was observed up to 8 weeks at 4 &deg;C and declined with elapse of storage duration. Facilitated extrusion of shoot from gel seed and pre-treatment of synthetic seeds with 100 mM KNO3for 5 minutes improved the regeneration frequency while mechanical removal of gel bead resulted in complete loss of explant viability.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=Biot-16-0049"
}]