[{
  "type": "article-journal",
  "title": "Antimicrobial Activity and Component Analysis of Cleome spinosa against Fusarium oxysporum",
  "author": [
    {
      "family": "Zhang",
      "given": ""
    },
    {
      "family": "Wang",
      "given": ""
    },
    {
      "family": "Liu",
      "given": ""
    },
    {
      "family": "Sa",
      "given": ""
    },
    {
      "family": "Gao",
      "given": ""
    },
    {
      "family": "Liu",
      "given": ""
    },
    {
      "family": "Liu",
      "given": ""
    },
    {
      "family": "Yang",
      "given": ""
    },
    {
      "family": "Ma",
      "given": ""
    },
    {
      "family": "Li",
      "given": ""
    },
    {
      "family": "Chen",
      "given": ""
    },
    {
      "family": "Du",
      "given": ""
    },
    {
      "family": "Zhang1",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2021]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "31",
  "issue": "5",
  "page": "1427-1438",
  "DOI": "https://doi.org/10.36899/JAPS.2021.5.0344",
  "abstract": "<p>Cucumber Fusarium wilt is an important soil-borne disease that restricts cucumber production in all areas of the world. To explore the preventive effects of<em>&nbsp;Cleome spinosa&nbsp;</em>on cucumber Fusarium wilt, five different doses of&nbsp;<em>Cleome spinosa&nbsp;</em>powder including 0, 0.1, 0.2, 0.3, and 0.4 g/dish were applied to cucumber plant infected with&nbsp;<em>Fusarium oxysporum&nbsp;</em>f.sp.<em>&nbsp;cucumarinum</em>at Northeast Agricultural University. The data thus collected on various parameters were subjected to one-way analysis of variance (ANOVA) under Completely Randomized Design (CRD). The difference in treatment means were separated using Duncan&rsquo;s Multiple Range (DMR) Test. A 100% inhibition rate on&nbsp;<em>F. oxysporum</em>&nbsp;mycelium was achieved when the dose of&nbsp;<em>C. spinosa&nbsp;</em>powder rose to 0.3 or 0.4 g/dish. As the concentration of the&nbsp;<em>Cleome&nbsp;</em><em>spinosa</em>&nbsp;extract increased, the inhibitory effects on diameters and dry weight of mycelium also increased. Median inhibitory concentration of&nbsp;<em>C. spinosa&nbsp;</em>on mycelium was was 45.12 mg mL<sup>-1</sup>.Gas chromatography-mass spectrometry identified twenty-one sorts of volatile constituents of&nbsp;<em>Cleome spinosa</em>, including heterocyclic compounds, alcohol, chromene, ester, acid and long chain alkanes. Twelve components of&nbsp;<em>F. oxysporum&nbsp;</em>were extracted by fumigation. Among those components, paeonol, linalool and theaspirane had antimicrobial activities against&nbsp;<em>F. oxysporum</em>, with inhibition rates of 73.9%, 75.9% and 80.4%, respectively. However, tetradecyl-oxirane, tetracosane, heptacosane, 3-ethyl-2-hydroxy-2-cyclopenten-1-one, octacosane, 1,1,4A-trimethyl-3,4,4A,5,6,7-hexahydro-1H-naphthalen-2-one,6,10,14-trimethyl-2-pentadecanone, methyl alpha-linolenate and pentatriacont-17-ene had no inhibitory effect against&nbsp;<em>F.</em><em>&nbsp;oxysporum</em>. Those results suggested that the components of&nbsp;<em>Cleome spinosa&nbsp;</em>powder could effectively restrain cucumber Fusarium wilt.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=BIOCH-20-0013"
}]