[{
  "type": "article-journal",
  "title": "PECTIN LYASE PRODUCTIVITY BY A UV-IRRADIATED ASPERGILLUS ORYZAE MUTANT UNDER CARROT-KOJI PROCESS",
  "author": [
    {
      "family": "Mahboob",
      "given": ""
    },
    {
      "family": "Ali",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2022]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "32",
  "issue": "5",
  "page": "1375-1384",
  "DOI": "https://doi.org/10.36899/JAPS.2022.5.0544",
  "abstract": "<p>Pectin lyase has several applications in different industrial areas. The enzyme has been produced by batch fermentation while the production of mutant pectin lyase has been improved by using carrot-koji fermentation. The present work deals with the stimulation in pectin lyase activity by a UV-irradiated&nbsp;<em>Aspergillus oryzae</em>&nbsp;mutant-auxotroph under carrot-koji process. The physical mutagenesis was induced using ultraviolet radiations. The effect of different distance from UV source (5-30 cm) and different exposure time (10-60 min) was investigated. The final mutant derivative UV-t30 was able to produce 9.26 U/ml of pectin lyase which was significantly higher than the wild-type. Two stage submerged fermentation was carried out while using carrot peelings as a substrate. The wild-type ISL-9 and mutant strain UV-t30 of&nbsp;<em>A. oryzae</em>&nbsp;showed the highest production by using 2 and 1.5 g carrot peelings, respectively 48 h after incubation (seeded with 8% inoculum). The enzyme was activated by CaCl<sub>2</sub>&nbsp;and (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>&nbsp;whereas it was inhibited in the presence of Tween-80. The enzyme was further activated by mutant strain UV-t30 while inhibited by wild-type ISL-9 using KNO<sub>3</sub>. After optimization of parameters for enzyme activity, the potent mutant showed a 1.3-fold increase in the enzyme activity as compared to the wild-type. The study proved that carrot peel has nutrients which enabled&nbsp;<em>A. oryzae</em> to produce pectin lyase in koji process.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=AS-20-0108"
}]