[{
  "type": "article-journal",
  "title": "PHYLOGENETIC RELATIONSHIP AMONG SELECTED SPECIES OF LAMIACEAE INFERRED FROM CHLOROPLAST RPS14 GENE",
  "author": [
    {
      "family": "Malik1",
      "given": ""
    },
    {
      "family": "Shahzad",
      "given": ""
    },
    {
      "family": "Arif",
      "given": ""
    },
    {
      "family": "Akhtar",
      "given": ""
    },
    {
      "family": "Mahmood",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2020]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "31",
  "issue": "4",
  "page": "1060-1069",
  "DOI": "https://doi.org/10.36899/JAPS.2021.4.0303",
  "abstract": "<p>The&nbsp;<em>Lamiaceae</em>&nbsp;is commonly known as Mint family of order Lamiales. The present study was conducted for assessment of genetic diversity, phylogeny of&nbsp;<em>Lamiaceae&nbsp;</em>based on&nbsp;<em>rps</em>14 gene and validation of&nbsp;<em>rps</em>14 protein by Ramachandran plots. The&nbsp;<em>rps</em>14 gene was amplified and sequence analyzed through MEGA7, I-TASSER and RAMPAGE. The phylogram revealed genetic distance (number of base substitutions per site) of 0.0100 among&nbsp;<em>Lamiaceae</em>&nbsp;species. In Pairwise distance, the range of genetic diversity was 0.013 to 0.089 with a mean of 0.060.&nbsp;<em>Vitex agnus castus&nbsp;</em>and<em>&nbsp;Vitex trifolia</em>&nbsp;were grouped in same cluster with Bootstrap value of 99 %.&nbsp;<em>Caryopteris odorata&nbsp;</em>revealed close relationship with<em>&nbsp;Volkameria inermis, Clerodendrum</em><em>calamitosum&nbsp;</em>and<em>&nbsp;Clerodendrum indicum&nbsp;</em>in same cluster having BS of 58 %.&nbsp;<em>C. indicum, C. calamitosum&nbsp;</em>and<em>&nbsp;V. inermis</em>&nbsp;showed close relationship in same cluster with BS of 51%. The 3D protein model designed by I-TASSER and its validation by RAMPAGE revealed &le; 2 % amino acid in outlier regions of&nbsp;<em>Gmelina philippensis</em>,&nbsp;<em>Clerodendrum. umbellatum</em>&nbsp;and&nbsp;<em>C. calamitosum</em>. This study concluded close genetic relationship and low genetic diversity that indicated potential of&nbsp;<em>rps</em>14 gene for phylogeny and diversity analysis among&nbsp;<em>Lamiaceae&nbsp;</em>species. The validation of 3D protein models by RAMPAGE showed good protein models. This study highlights the evolutionary relationship, earliest, recent evolution of&nbsp;<em>Lamiaceae&nbsp;</em>species. A very low genetic diversity indicates ex-situ conservation and good protein models in&nbsp;<em>Lamiaceae&nbsp;</em>species.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=AG-20-0072"
}]