[{
  "type": "article-journal",
  "title": "ROSMARINIC ACID MITIGATES RADIATION-INDUCED THYMIC AND SPLENIC INJURY THROUGH ANTIOXIDANT ENHANCEMENT AND MODULATION OF THYMIC mRNA EXPRESSION IN MICE",
  "author": [
    {
      "family": "Rashid",
      "given": ""
    },
    {
      "family": "Matloob",
      "given": ""
    },
    {
      "family": "Rauf",
      "given": ""
    },
    {
      "family": "Asad",
      "given": ""
    },
    {
      "family": "Uddin",
      "given": ""
    },
    {
      "family": "Shah",
      "given": ""
    },
    {
      "family": "Khan",
      "given": ""
    },
    {
      "family": "Ikram",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2027]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "37",
  "issue": "1",
  "DOI": "https://doi.org/10.36899/JAPS.2027.1.0013",
  "abstract": "<p style=\"text-align: justify;\">Ionizing radiation causes significant harm to lymphoid organs through oxidative stress, cellular depletion, and disruption of tissue architecture. The study aimed to establish a sublethal&nbsp;<sup>137</sup>Cs &gamma;-radiation-induced model of thymic and splenic injury and to evaluate the mitigating effects of rosmarinic Acid (RA). Total 144 BALB/c mice, 6-8 weeks age and 18-22 g weight, were randomly allocated into six groups: normal control, irradiated control, RA50, RA100, RA150, and levamisole. Mice were exposed to&nbsp;<sup>137</sup>Cs &gamma;-radiation to induce thymic and splenic injury and were subsequently treated with RA at doses of 50, 100, 150 mg/kg for 14 days. The mitigating effects of RA were assessed using hematological parameters, thymic and splenic indices, organ cellularity, histological architecture, oxidative-stress and antioxidant markers, and the thymic expression of FoxN1, IL-6, IL-7, and IL-15. At 150 mg/kg dose, thymus and spleen index increased significantly (p &le; 0.001) from irradiated group. Histological examination showed reduced radiation-associated hypocellularity and architectural disruption in the thymus and spleen, improvement observed at 150 mg/kg. RA significantly reduced malondialdehyde levels in the thymus and spleen (p &le; 0.001) than irradiated group following treatment with 150mg/kg and significantly improved SOD (p &le; 0.001), GPx (p &le; 0.001), CAT (p &le; 0.01) activity. Furthermore, RA significantly modulated the radiation-altered expression of IL-7 (p &le; 0.001), IL-6, IL-15, and FoxN1 (p &le; 0.01) mRNA in the thymus, at 150 mg/kg. Overall, RA, mitigated sublethal radiation-associated thymic and splenic injury, as demonstrated by improvements in hematological and organ indices, oxidative-stress and antioxidant markers, lymphoid tissue architecture, and thymic mRNA expression. These findings suggest that RA may have potential as a natural immunomodulatory agent against radiation-associated lymphoid injury.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2026-JAPS-648"
}]