[{
  "type": "article-journal",
  "title": "Diverse expression of isoflavonoid-related genes based on transcriptomic datasets of Pueraria mirifica cultivars",
  "author": [
    {
      "family": "Hue",
      "given": ""
    },
    {
      "family": "Ngoc",
      "given": ""
    },
    {
      "family": "Ha",
      "given": ""
    },
    {
      "family": "Pham",
      "given": ""
    },
    {
      "family": "Nguyen",
      "given": ""
    },
    {
      "family": "Nguyen",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2026]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "36",
  "issue": "4",
  "DOI": "https://doi.org/10.36899/JAPS.2026.4.0100",
  "abstract": "<p class=\"Keyword\" style=\"margin-top: 12pt; line-height: normal; text-align: justify;\"><span lang=\"EN-GB\" style=\"mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'Times New Roman'; color: windowtext; mso-ansi-language: EN-GB;\">White Kwao Krua, also known as <em>Pueraria mirifica</em>, is a traditional medicinal plant in several Asian countries. This plant has a high content of essential phytoestrogens such as isoflavones and chromenes, particularly miroestrol. However, </span><span style=\"mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'Times New Roman'; color: windowtext;\">their biosynthetic pathway remains unclear and is currently under investigation</span><span lang=\"EN-GB\" style=\"mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'Times New Roman'; color: windowtext; mso-ansi-language: EN-GB;\">. Three gene families Chalcone isomerases (<em>CHI</em>s), Chalcone synthases (<em>CHSs</em>), and </span><span lang=\"TR\" style=\"mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'Times New Roman'; color: windowtext; mso-ansi-language: TR;\">UDP-dependent glycosyltransferases</span><span lang=\"TR\" style=\"mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'Times New Roman'; color: windowtext; mso-ansi-language: EN-GB;\"> </span><span lang=\"EN-GB\" style=\"mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'Times New Roman'; color: windowtext; mso-ansi-language: EN-GB;\">(<em>UGTs</em>) play a key role in the phytoestrogen biosynthesis in <em>P. mirifica</em>. They are large gene families with myriad members, involved in several plant functions. In the research, five <em>P. mirifica</em> cultivars (</span><span style=\"mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'Times New Roman'; color: windowtext;\">TLBYT, TLCNX, TLDB, NA, and SL)</span><span style=\"mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'Times New Roman'; color: windowtext; mso-ansi-language: EN-GB;\"> <span lang=\"EN-GB\">were cultivated under the same conditions and then their leaf, stem, and tuber tissues were collected. The transcriptomes of the cultivars were sequenced, assembled, and annotated in the research. </span></span><span lang=\"TR\" style=\"mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'Times New Roman'; color: windowtext; mso-ansi-language: TR;\">By using RNA-seq, the t</span><span style=\"mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'Times New Roman'; color: windowtext;\">ranscriptome assembly yielded over 300,000 unigenes, of which approximately 32,000 were annotated across four major databases:<em> </em>NCBI-Nr (229569 unigenes), SwissProt (158667 unigenes), COG (112089 unigenes), and KEGG (61480 unigenes). </span><span lang=\"EN-GB\" style=\"mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'Times New Roman'; color: windowtext; mso-ansi-language: EN-GB;\">Seventeen individuals from these gene families that may catalyze or influence miroestrol and isoflavone biosynthesis were detected. The RT-qPCR analysis revealed tissue-specific gene expression, with several genes showing preferential expression in either leaves or tubers. <em>CHS11</em>, <em>CHS13</em>, and <em>UGT74</em> gene were predominantly expressed in leaves, whereas <em>CHI4A</em>, <em>CHI3A2</em>, and <em>CHS14</em> showed higher expression in tubers, the primary site of phytoestrogen accumulation. These results provide transcriptomic data for different <em>P. mirifica</em> varieties and demonstrate tissue-specific expression patterns of key <em>CHI</em>, <em>CHS</em>, and <em>UGT</em> genes involved in the isoflavonoid biosynthetic pathway.</span></p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2025-JAPS-770"
}]