[{
  "type": "article-journal",
  "title": "IDENTIFICATION OF THREATENED AND ENDEMIC ANGELICA GLAUCA: INSIGHT FROM DNA BARCODING AND PHYLOGENETIC ANALYSIS",
  "author": [
    {
      "family": "Sajjad",
      "given": ""
    },
    {
      "family": "Muhammad",
      "given": ""
    },
    {
      "family": "Islam",
      "given": ""
    },
    {
      "family": "Ali",
      "given": ""
    },
    {
      "family": "Umer",
      "given": ""
    },
    {
      "family": "Azeem",
      "given": ""
    },
    {
      "family": "Rehman",
      "given": ""
    },
    {
      "family": "Ullah",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2026]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "36",
  "issue": "2",
  "page": "530-541",
  "DOI": "https://doi.org/10.36899/JAPS.2026.2.0043",
  "abstract": "<p><em>Angelica glauca&nbsp;</em>Edgew is a perennial non-vascular herb belonging to the family Apiaceae, having a high medicinal and economic value. However,&nbsp;<em>A. glauca</em>&nbsp;faces threats from climate change, habitat loss, and overharvesting, which reduce its natural populations. To address these issues, the DNA barcoding technique was employed as a taxonomic tool to confirm the accurate identification and support the conservation of this species. 2% CTAB was used to extract total genomic DNA, andDNA barcode markers such as (ITS2,&nbsp;<em>matK, rbcL,</em>&nbsp;and&nbsp;<em>rpoB</em>) were amplified and sequenced. Nucleotide sequence analysis of Angelica species revealed that the conserved, parsimony-informative, and singleton sites vary from primer to primer. The highest number of variable sites was recorded at 192 in the&nbsp;<em>matK</em>&nbsp;marker, followed by 170 in the&nbsp;<em>rpoB</em>&nbsp;marker. A phylogenetic tree was generated through the neighbour-joining method. It showed that&nbsp;<em>A. glauca,</em>&nbsp;based on the ITS2 sequence (LC860493), was closely clustered with&nbsp;<em>A. archangelica-</em>MT735413.1, based on&nbsp;<em>matK</em>&nbsp;(LC860494), with&nbsp;<em>A. archangelica</em>-GQ248079.1 based on&nbsp;<em>rbcL&nbsp;</em>(LC860492), with&nbsp;<em>A. archangelica</em>-GQ248549.1, and based on&nbsp;<em>rpoB</em>&nbsp;(LC593680) with&nbsp;<em>A. megaphylla</em>-NC057128.1, with bootstrap support of 63, 70, 100, and 43, respectively. The present study concluded that&nbsp;<em>A. glauca</em>&nbsp;is closely related to&nbsp;<em>A. archangelica</em>&nbsp;within the genus&nbsp;<em>Angelica</em>, and the markers used in this study are recommended for improved identification and conservation of other threatened or endangered plant species.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2025-JAPS-235"
}]