[{
  "type": "article-journal",
  "title": "MULBERROSIDE A EXTRACT ENHANCES THE ANTITUMOR EFFICACY OF PACLITAXEL BY INHIBITING THE HIF-1Α/SHH SIGNALING PATHWAY IN LEWIS LUNG CARCINOMA-BEARING MICE",
  "author": [
    {
      "family": "Liu",
      "given": ""
    },
    {
      "family": "Yan",
      "given": ""
    },
    {
      "family": "Jiang",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2026]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "36",
  "issue": "6",
  "DOI": "https://doi.org/10.36899/JAPS.2026.6.0149",
  "abstract": "<p style=\"text-align: justify;\">The present work set out to determine whether Mulberroside A (MA) extract could boost the antitumor efficacy of paclitaxel (PTX) in Lewis lung carcinoma (LLC)-bearing mice through modulation of the HIF-1&alpha;/SHH signaling pathway. LLC-bearing C57BL/6 mice were randomized into control, model, PTX, MA, PTX+MA, PTX+MA+HIF-1&alpha; inhibitor (YC-1) and PTX+MA+SHH inhibitor (Cyclopamine) groups. Tumor growth, apoptosis (TUNEL), microvascular density (MVD and CD31 staining), and histopathology (H&amp;E) were assessed. Expression of HIF-1&alpha;/SHH pathway components and downstream targets (VEGF, Bcl-2 and caspase-3) was measured by qPCR, and Western blot.&nbsp;&nbsp;PTX+MA synergistically inhibited tumor growth and reduced MVD versus PTX alone (<em>P</em>&le;0.05), while a higher level of apoptotic cells was observed (<em>P</em>&le;0.05). This was accompanied by downregulation of HIF-1&alpha;, SHH, SMO, GLI1, VEGF, and Bcl-2, and upregulation of caspase-3 (<em>P</em>&le;0.05). Addition of HIF-1&alpha; or SHH inhibitors partially reversed these effects, confirming pathway involvement. MA enhances PTX antitumor efficacy in LLC-bearing mice by suppressing the HIF-1&alpha;/SHH axis, thereby inhibiting angiogenesis and promoting apoptosis. This combination may represent a potential strategy to improve chemotherapy efficacy in lung cancer.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2025-JAPS-1366"
}]