[{
  "type": "article-journal",
  "title": "EFFECT OF TEMPERATURE ON PRODUCTION OF Moina brachiate AS LIVE FEED FOR Hypophthalmichthys molitrix FRY",
  "author": [
    {
      "family": "Mirza",
      "given": ""
    },
    {
      "family": "Rashid",
      "given": ""
    },
    {
      "family": "Shafi",
      "given": ""
    },
    {
      "family": "Shakeeb",
      "given": ""
    },
    {
      "family": "Saeed",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2025]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "35",
  "issue": "3",
  "page": "681-688",
  "DOI": "https://doi.org/10.36899/JAPS.2025.3.0057",
  "abstract": "<p>The lack of mass culture techniques for&nbsp;<em>Moina brachiate</em>&nbsp;limits its use as a sustainable live feed in aquaculture. Present study was conducted to culture&nbsp;<em>M. brachiate&nbsp;</em>under indoor conditions at different temperatures and use it as live feed for&nbsp;<em>H</em><em>ypophthalmichthys molitrix</em>fry. The isolated&nbsp;<em>M. brachiate</em>&nbsp;was cultured in the laboratory for 10 days in glass aquaria under two experimental treatments in which water temperature was maintained either at 28.5&deg; &plusmn; 1.5 &deg;C (T1) or 23.5&deg; &plusmn; 1.5 &deg;C (T2).&nbsp;<em>Chlorella vulgaris&nbsp;</em>was used to feed&nbsp;<em>M. brachiate&nbsp;</em>daily and the feed consumption was regularly monitored. Chemical quality parameters of the water were regularly monitored and kept within suitable range for propagationof&nbsp;<em>Moina</em>. At the end of the culture period, the maximum density of&nbsp;<em>M. brachiate</em>&nbsp;was found in T2 (5.92 individuals/ ml). In T1 (83.05 &plusmn; 1.39%) a decrease in density of&nbsp;<em>M. brachiate</em>&nbsp;density was observed compared to its initial count. Consumption of&nbsp;<em>C. vulgaris</em>&nbsp;by&nbsp;<em>M. brachiate&nbsp;</em>was significantly high in T2 compared to T1 (90.54 &plusmn; 3.69% and 69.32 &plusmn; 11.81% respectively). Cysts of&nbsp;<em>M. brachiate</em>&nbsp;were observed to be formed under drastic environmental conditions with hatching time of 7-8 days under optimum treatment. In the second phase of the experiment,&nbsp;<em>M. brachiate</em>&nbsp;was used to feed&nbsp;<em>H. molitrix&nbsp;</em>fry reared in glass aquaria and resulted in 86.0% survival of fry. The findings demonstrate the significant influence of temperature on&nbsp;<em>M. brachiate</em>&nbsp;propagation and suggest that its culture at&nbsp;<strong>23.5&deg; &plusmn; 1.5 &deg;C water temperature with daily feeding of&nbsp;<em>Chlorella vulgaris</em></strong><strong>,&nbsp;</strong>can be a viable live feed option for&nbsp;<em>H. molitrix&nbsp;</em>fry in aquaculture.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2024-JAPS-1788"
}]