[{
  "type": "article-journal",
  "title": "PURIFICATION, CHARACTERIZATION OF GLUTATHIONE S-TRANSFERASE FROM THE LIVER TISSUE OF GOOSE (Anser anser Domesticus) AND INHIBITION EFFECTS OF SOME METAL IONS AND CHEMICALS",
  "author": [
    {
      "family": "SAYIN",
      "given": ""
    },
    {
      "family": "YERLIKAYA",
      "given": ""
    },
    {
      "family": "DEMIRDAG",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2024]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "34",
  "issue": "5",
  "page": "1250-1257",
  "DOI": "https://doi.org/10.36899/JAPS.2024.5.0807",
  "abstract": "<p><span lang=\"EN-GB\">The GST enzyme is an antioxidant that protects organs against toxic substances. It reduces reactive oxygen species to less reactive metabolites.</span><span lang=\"EN-GB\">The GST enzyme has been studied in many living creatures such as sheep, birds, fish, bacteria, cattle, plants and humans. However, no studies on the Goose (<em>Anser anser Domesticus</em>) liver GST enzyme (E.C. 2.5.1.18) have been found in the literature. This study demonstrates the purification and characterization of the GST enzyme from Goose (<em>Anser anser Domesticus</em>) liver tissue by affinity chromatography. Besides, inhibitory effects of&nbsp;</span><span lang=\"TR\">Hg<sup>2+</sup>, Ag<sup>2+</sup>&nbsp;and Fe<sup>2+&nbsp;</sup></span><span lang=\"TR\">cations</span><span lang=\"EN-GB\">&nbsp;and Oxytetracycline HCl, Tylosin Tartrate, Enrofloxacin and Doxycycline Hyclate chemicals on GST enzyme activity were investigated. The research was conducted under&nbsp;<em>in vitro</em>&nbsp;conditions using 1-Chloro-2,4-dinitrobenzene method. The degree of purity of the enzyme solution was verified by SDS-PAGE. With the characterization of the enzyme, the optimal pH, optimal ionic strength and optimal temperature values were found to be 7.0, 100 mM and 40 &deg;C, respectively. Separate i</span><span lang=\"TR\">nhibition graphs of Hg<sup>2+</sup>, Ag<sup>2+</sup>, Fe<sup>2+</sup>, Oxytetracycline HCl, Tylosin Tartrate, Enrofloxacin and Doxycycline Hyclate inhibitors were drawn. IC<sub>50</sub>&nbsp;levels of inhibitors were found from the Activity% - [Inhibitor] graphs. As a result, the inhibition order of the inhibitors was found to be Hg<sup>2+</sup></span><span lang=\"EN\">&nbsp;&gt;&nbsp;</span><span lang=\"TR\">Ag<sup>2+</sup></span><span lang=\"EN\">&nbsp;&gt; F</span><span lang=\"TR\">e<sup>2+&nbsp;</sup></span><span lang=\"TR\">for cations, and Enrofloxacin ˂ Tylosin Tartrate ˂ Doxycycline Hyclate ˂ Oxytetracycline HCl for chemicals.</span></p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2024-JAPS-1677"
}]