[{
  "type": "article-journal",
  "title": "SNAKE VENOM ENZYMES NEUTRALIZATION POTENTIAL OF PHYTOCHEMICALS EXTRACTED FROM DATURA ALBA SEEDS",
  "author": [
    {
      "family": "Fatima",
      "given": ""
    },
    {
      "family": "Tahir",
      "given": ""
    },
    {
      "family": "Moin",
      "given": ""
    },
    {
      "family": "Waheed",
      "given": ""
    },
    {
      "family": "Khan",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2024]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "34",
  "issue": "5",
  "page": "1112-1118",
  "DOI": "https://doi.org/10.36899/JAPS.2024.5.0793",
  "abstract": "<p><em>Datura</em>&nbsp;is a flowering perennial herb, belonging to the family Solanaceae, is widely distributed in many countries including Pakistan, and other tropical and sub-tropical parts of the world. The present study explored the bioactivities of phytochemicals obtained from&nbsp;<em>Datura alba</em>&nbsp;n-hexane and n-butanol seed extracts. The seed extracts showed the presence of flavonoids, terpenoids and alkaloids while saponins were absent. The n-hexane extract showed 100% human salivary amylase and 19% snake venom PLA<sub>2</sub>&nbsp;(svPLA<sub>2</sub>) inhibition activity at examined lowest and highest concentrations respectively. It also exhibited mild (24% RSA) antioxidant activity at highest concentration (600 &micro;g/&micro;l) analyzed. On the other hand, salivary amylase inhibition and potent (80% RSA) antioxidant activity at 100 &micro;g/&micro;l concentration was observed in n-butanol extract. Interestingly, n-hexane extract showed snake venom protease while n-butanol extract showed svPLA<sub>2</sub>&nbsp;enhancing activity and no venom anti-protease activity. The study demonstrated that both n-hexane and n-butanol extracts are natural sources of antioxidant and antidiabetic compounds. The n-hexane extract showed venom PLA<sub>2</sub> neutralization which is reported for the first time.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2023-JAPS-905"
}]