[{
  "type": "article-journal",
  "title": "EFFECTS OF SOIL SALT CONCENTRATION ON nirS AND nosZ GENES DIVERSITY OF PADDY SOIL DENITRIFYING BACTERIA",
  "author": [
    {
      "family": "Ding",
      "given": ""
    },
    {
      "family": "He",
      "given": ""
    },
    {
      "family": "Wu",
      "given": ""
    },
    {
      "family": "Li",
      "given": ""
    },
    {
      "family": "Liu",
      "given": ""
    },
    {
      "family": "Liao",
      "given": ""
    },
    {
      "family": "Zhou",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2023]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "33",
  "issue": "2",
  "page": "270-277",
  "DOI": "https://doi.org/10.36899/JAPS.2023.2.0618",
  "abstract": "<p>To further study saline-alkali soil cultivation, the terminal-restriction fragment length polymorphism (T-RFLP) was used to analyze the effects of different salt concentrations on the denitrification functional genes&nbsp;<em>nirS</em>&nbsp;and<em>&nbsp;nosZ</em>&nbsp;after collecting different soil samples and extracting total soil DNA. The results showed that the variation range of&nbsp;<em>nirS</em>&nbsp;gene abundance was concentrated in 14 segments, the&nbsp;<em>nosZ</em>&nbsp;gene abundance was concentrated in 9 segments. The abundance of&nbsp;<em>nirS</em>&nbsp;gene in paddy soil of different concentrations was higher than&nbsp;<em>nosZ</em>, and the activity of the&nbsp;<em>nirS</em>&nbsp;gene was higher. Diversity index analysis showed that sample Ⅰ was significantly different from Ⅲ and Ⅴ in the Shannon index of the&nbsp;<em>nirS</em>. Sample Ⅰ and Ⅳ were significantly different from Ⅲ in Simpson index. Except for the significant difference between Ⅲ and Ⅰ, Ⅱ, Ⅳ, and Ⅴ, there was no difference between the other four samples significant in Pielou index. The difference between sampl Ⅰ and Ⅴ was extremely significant in Brillouin index. The Shannon index, Simpson index and Pielou index of&nbsp;<em>nosZ</em>&nbsp;gene were not significantly different, sample Ⅱ and Ⅲ, Ⅳ, Ⅴ were significantly different in Brillouin index. The diversity of&nbsp;<em>nirS</em>&nbsp;gene was more affected by the salt concentration, and the&nbsp;<em>nirS</em>&nbsp;gene was more widely distributed than&nbsp;<em>nosZ</em>&nbsp;in soil with different salt concentrations. It indicated that&nbsp;<em>nirS</em> -mediated nitrite reductase played an important role in salinity affected soil environment.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2022-JAPS-90"
}]