[{
  "type": "article-journal",
  "title": "ISOLATION AND STRUCTURAL ELUCIDATION OF PHENOLIC COMPONENTS FROM CALOTROPIS PROCERA (AIT) AND EVALUATION OF INSECTICIDAL ACTIVITY",
  "author": [
    {
      "family": "Nacira",
      "given": ""
    },
    {
      "family": "Mebarka",
      "given": ""
    },
    {
      "family": "Yacine",
      "given": ""
    },
    {
      "family": "Mohamed",
      "given": ""
    },
    {
      "family": "Roukia",
      "given": ""
    },
    {
      "family": "Mahfoud",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2023]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "33",
  "issue": "4",
  "page": "971-977",
  "DOI": "https://doi.org/10.36899/JAPS.2023.4.0690",
  "abstract": "<p>The identification and characterization of two phenolic compounds from the ethyl acetate (EtAc) extract of&nbsp;<em>Calotropis procera&nbsp;</em>aerial parts were performed using chromatographic and spectroscopic methods namely Thin Layer Chromatography (TLC), Preparative Thin Layer Chromatography (PTLC), High-Performance Liquid Chromatography (HPLC), Infrared spectroscopy (FT-IR) and Nuclear Magnetic Resonance (<sup>1</sup>H-NMR,&nbsp;<sup>13</sup>C-NMR).Two major compounds were isolated to be the 3, 3&rsquo;, 4&rsquo;, 5, 7-pentahydroxy flavones-3-rutinoside (Rutin) and 3,4,5-trihydroxybenzoic acid(Gallic acid), the results were confirmed by comparison with standards. In addition, the insecticidal activity of these isolated compounds was determined against&nbsp;<em>Parlatoria blanchardi</em><em>targ&nbsp;</em>individuals, 71.73% and 59.44% mortality were recorded at 0.5 mg/ml of Rutin and Gallic acid respectively, after 24 h of treatment; whereas, the larval stages were the most sensitive to Rutin treatment (LD<sub>50&nbsp;</sub>equal to0.10 mg/ml for 1<sup>st&nbsp;</sup>larva and 0.18 mg/ml for 2<sup>nd</sup> larva).</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2022-JAPS-612"
}]