[{
  "type": "article-journal",
  "title": "POOLED MAPPING OF QUANTITATIVETRAIT LOCI ASSOCIATED WITH DROUGHT TOLERANCE IN RICE (ORYZA SATIVA L.) AT SEEDLING STAGE",
  "author": [
    {
      "family": "Wu",
      "given": ""
    },
    {
      "family": "Zhang",
      "given": ""
    },
    {
      "family": "Zhang",
      "given": ""
    },
    {
      "family": "Tang",
      "given": ""
    },
    {
      "family": "Wang",
      "given": ""
    },
    {
      "family": "Zhang",
      "given": ""
    },
    {
      "family": "Wu",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2023]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "33",
  "issue": "6",
  "page": "1467-1473",
  "DOI": "https://doi.org/10.36899/JAPS.2023.6.0686",
  "abstract": "<p><span lang=\"EN-US\">Drought is among the foremost constraints influencing global rice productivity. The drought tolerance nature of rice is complicated, depending on multiple components and having low heritability. Thus, breeding drought-tolerant varieties is a fundamental way which can be used to increase rice yield in drought. To investigate the genetic basis of seedling tolerance to drought stress of rice (<em>Oryza sativa&nbsp;</em>L.), we performed QTL mapping on a big F<sub>2</sub>&nbsp;population of 2600 participants from a cross between the&nbsp;<em>japonica</em>&nbsp;rice variety Huaidao 5 (HD5) and the&nbsp;<em>indica&nbsp;</em>rice variety 1892S&nbsp;</span><span lang=\"EN-US\">through performing bulked segregant analysis and whole-genome sequencing (BSA-seq).</span><span lang=\"EN-US\">&nbsp;HD5 showed greater tolerance to prolonged drought stress compared with1892S</span><span lang=\"EN-US\">at seedling stage. By analyzing a pair of opposite DNA pools made from 182 extremely-sensitive seedlings and 182 extremely-tolerant seedlings from the F<sub>2</sub>&nbsp;population using the block regression mapping (BRM) method, we mapped a QTL on chromosome 1, of which the additive effect was estimated to explain 2.20% of the phenotypic variance. We named the QTL</span><em><span lang=\"EN-US\">qSLDT1.1</span></em><span lang=\"EN-US\">(<em>q</em>&nbsp;represents quantitative trait loci,&nbsp;<em>SL&nbsp;</em>represents seedling leaf,&nbsp;<em>DT&nbsp;</em>represents drought tolerance,&nbsp;<em>1.1</em></span><span lang=\"EN-US\">representsthe first one found on chromosome 1</span><span lang=\"EN-US\">), which must be a novel QTL, because no QTLs for rice seedling tolerance to drought stress have been mapped on chromosome 1 before. The information derived from the current research facilitates marker-assisted breeding of drought-resistant lines and positional cloning of the gene conferring drought tolerance in rice.</span></p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2022-JAPS-490"
}]