[{
  "type": "article-journal",
  "title": "EVALUATION OF THE PHYTOCHEMICAL PROPERTIES AND DNA PROTECTIVE ACTIVITY OF BOLANTHUS ORTEGIOIDES (FISCH. and C.A. MEY.) MADHANI and RABELER",
  "author": [
    {
      "family": "Sönmez",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2023]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "33",
  "issue": "2",
  "page": "425-433",
  "DOI": "10.36899/JAPS.2023.2.0634",
  "abstract": "<p>The aim of this study was to elucidate the potential of the flora of Turkey by investigating the phytochemical properties of the endemic plant species&nbsp;<em>Bolanthus ortegioides</em>&nbsp;of the family Caryophyllaceae. The identity of the plant was validated through a phylogenetic approach with DNA barcoding using the internal transcribed spacer [ITS] region. Methanolic extract of&nbsp;<em>B. ortegioides</em>&nbsp;was examined by HPLC, and the total phenolic content was measured. In addition, pBluescript II SK(+) plasmid DNA cleavage was induced by ultraviolet (UV) photolysis of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), and the activity of the methanolic extract of&nbsp;<em>B. ortegioides</em>&nbsp;against oxidative DNA damage was examined. The total phenolic content of the extract was 5.6 &plusmn; 0.25 &micro;g (gallic acid equivalent [GAE])/mg. Of 15 phenolic compounds analysed by HPLC, vanillic acid was found to be a major compound. Chlorogenic, caffeic, epicatechin, p-coumaric, ferulic, rutin and chicoric acids were also detected. The results showed that&nbsp;<em>B. ortegioides</em> methanolic extract offered moderate protection against DNA damage.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2022-JAPS-396"
}]