[{
  "type": "article-journal",
  "title": "EVALUATION OF IMMUNOGENICITY AND DOSE DEPENDENT IMMUNE RESPONSE AGAINST COMMON BOVINE ORIGINATED MASTITOGENS IN RABBIT MODEL",
  "author": [
    {
      "family": "Shah",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2023]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "33",
  "issue": "3",
  "page": "526-533",
  "DOI": "https://doi.org/10.36899/JAPS.2023.3.0645",
  "abstract": "<p>This study evaluated the immunogenicity and dose-dependent immune response of toxinotypes of bovine origin viz&nbsp;<em>Staphylococcus aureus (tst), Streptococcus uberis (</em>Cpn-60 targeted STUB<em>),&nbsp;</em>and<em>&nbsp;Escherichia coli (aggR)&nbsp;</em>in a rabbit model &ndash; a step forward towards an effective polyvalent mastitis vaccine. To evaluate the primary and secondary immune response (immunogenicity) against the concentration of 10<sup>6&nbsp;</sup>cells/mL of each preparation containing subject vaccinal isolates, 24 rabbits were divided randomly into 4 equal groups viz A, B, C and D. Each antigenic preparation was inoculated to the rabbits of group A to C @ 0.2 mL SC while group D served as control (placebo) at day 0 as priming dose and a booster dose at day 7 respectively. The serum antibodies titers were recorded in terms of Optical Density (OD) values at day 0 (pre-inoculation), 7, 14, 21 and 28 (post-inoculation) by iELISA Mastitis Kit (Abbexa, UK). The antibody response was significantly higher (p&lt;0.05) in the post-booster samples at day-14 and day-21 for all the selected isolates. The highest primary antibody response (1.74 &plusmn; 0.14) as well as secondary immune response (2.04 &plusmn; 0.13) was shown by&nbsp;<em>Strept. uberis</em>&nbsp;(Cpn-60 STUB) in group B on day-14 followed in order by group C and A respectively. Likewise, dose-dependent immune response to composite antigens of 3 different antigenic concentrations of vaccinal isolates: 10<sup>8&nbsp;</sup>cells/mL; 10<sup>10&nbsp;</sup>cells/mL; 10<sup>12</sup>&nbsp;cells/mL of each of vaccinal isolates in 18 adult rabbits divided randomly into 3 groups of 6 (A, B and C) respectively for dose standardization was evaluated. Serum samples were collected at weekly intervals following completion of 2<sup>nd</sup>&nbsp;shot of inocula of each dose for 3 consecutive weeks. The rabbits of group B injected with 10<sup>10</sup>&nbsp;cells mL<sup>-1</sup>&nbsp;of&nbsp;<em>S. aureus (tst)</em>&nbsp;showed a significantly higher (p&lt;0.05) serum iELISA O.D value (2.97<sup>A, a</sup>&nbsp;&plusmn; 0.10) followed in order by&nbsp;<em>E. coli (aggR)</em>&nbsp;(2.84<sup>a</sup>&nbsp;&plusmn; 0.08) and&nbsp;<em>Str. uberis (cpn60 STUB)</em>&nbsp;(2.78<sup>a</sup>&nbsp;&plusmn; 0.07) at day-14 post-inoculation. This study revealed the concentration of 10<sup>10</sup> cells/mL of each subject mastitogens as a standard bacterial load for polyvalent mastitis vaccines with higher and sustained antigenicity in rabbits.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2022-JAPS-374"
}]