[{
  "type": "article-journal",
  "title": "EXPRESSION OF TOMATO PATHOGENESIS RELATED GENES IN RESPONSE TO TOBACCO MOSAIC VIRUS",
  "author": [
    {
      "family": "Abdelkhalek",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2019]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "29",
  "issue": "6",
  "page": "1596-1602",
  "DOI": "NA",
  "abstract": "<p><em>Tobacco mosaic virus</em>&nbsp;(TMV) causes severe damages and economic losses of tomato crop production and quality worldwide. In the current study, the nucleotide sequence with NCBI-BLAST and phylogenetic analysis for the movement protein gene revealed that the Egyptian TMV isolate (Acc# MG264131) exhibited 99% identity with that of a Slovenian isolate (Acc# KY810785). Moreover, the transcription profiles of PR1, PR2, PR3, PR5 and PR7 genes in TMV-infected tomato tissues were analysed using semi-quantitative RT-PCR technique. Compared to mock-inoculated plants, the statistical analysis revealed significant changes in the relative expression level of both PR2 and PR7 at the two intervals 6 and 15 days, post-inoculation with 32.3 and 34.7-fold change respectively. A significant change in transcription level of the two genes suggests that they play an essential role in the tomato defence system and could be disease-specific genes induced with the TMV infection. Based on the data obtained in this study and the previous studies, the down-regulation of PR2 and/or overexpression of PR7 in tomato plants could provide new insights in developing efficient strategies for reducing the tomato susceptibility to TMV infection.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2019-JAPS-611"
}]