[{
  "type": "article-journal",
  "title": "PCR-BASED SCREENING OF PLASMODIUM SPECIES IN MOSQUITO VECTORS OF FAISALABAD DISTRICT, PUNJAB, PAKISTAN",
  "author": [
    {
      "family": "K.Hayat",
      "given": ""
    },
    {
      "family": "M.S.Sajid",
      "given": ""
    },
    {
      "family": "Z.Iqbal",
      "given": ""
    },
    {
      "family": "M.Saqib",
      "given": ""
    },
    {
      "family": "F.A.Raza",
      "given": ""
    },
    {
      "family": "W.Akram",
      "given": ""
    },
    {
      "family": "H.Abbas",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2019]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "29",
  "issue": "6",
  "page": "1568-1574",
  "DOI": "NA",
  "abstract": "<p><em>Plasmodium (P),</em>&nbsp;mosquito-borne unicellular parasite, is responsible for &ldquo;malaria&rdquo;. Pakistan remains at risk of malaria and almost 1.6 million cases of malaria are reported every year. The present study was planned to screen the mosquito vectors for&nbsp;<em>Plasmodium&nbsp;</em>sp. in Faisalabad district, Punjab, Pakistan using nested PCR. For this purpose, convenient sampling of adult mosquitoes was done from different places including: animal populated areas, lavatories, water storage tanks, livestock farms and road-side ditches in 70% ethanol. DNA extraction was done after stereomicroscopic identification of the specimens. Species identification of&nbsp;<em>P. falciparum, P. vivax, P. ovale</em>&nbsp;and&nbsp;<em>P. malariae</em>&nbsp;was done through universal forward and species-specific reverse primers in the nested PCR. Prevalence of&nbsp;<em>Culex&nbsp;</em>mosquitoes was higher as compared to&nbsp;<em>Anopheles</em>.&nbsp;<em>Plasmodium falciparum</em>&nbsp;and&nbsp;<em>P. vivax&nbsp;</em>were found higher as compared to other species of&nbsp;<em>Plasmodium</em>. The overall prevalence of&nbsp;<em>Plasmodium&nbsp;</em>sp. in mosquito vectors was 46% (14 out of 30 pools for&nbsp;<em>Plasmodium&nbsp;</em><em>sp.</em>). Results were analyzed through chi-square analyses. Present study may explore the vectorial capacity of mosquitoes which can be an indicator of&nbsp;<em>Plasmodium&nbsp;</em>sp. distribution in an area for large scale metagenomics.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2019-JAPS-607"
}]