[{
  "type": "article-journal",
  "title": "ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF A CHEMICALLY INDUCED MUTANT OF XANTHORIAPARIETINA",
  "author": [
    {
      "family": "Ali",
      "given": ""
    },
    {
      "family": "Hameed",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2019]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "29",
  "issue": "3",
  "page": "881-888",
  "DOI": "NA",
  "abstract": "<p>Biological active secondary metabolite parietin was extracted from wild-type and mutant strain of lichen&nbsp;<em>Xanthoriaparietina</em>. Six different solvents (acetone, ethanol, methanol, hexane, chloroform and distilled water) were used to extract parietin. The parietin activity was found to be maximum when extraction was carried out with 3 ml of methanol at 30&ordm;C for 1h. The extracts were proceeded to antibacterial activity against different strains of bacteria.Anion DPPH (1,1-Diphenyl-2-picrylhydrazyl) scavenging, reducing power and total flavonoid content were used to analyze the antioxidant activity. HPLC analysis represented more than 90% purity of methanol extract with some trace elements. The antioxidant activity increased with increasing concentration of methanol extract. EMS treated strain represented maximum DPPH scavenging activity i.e., 84&plusmn;3.36% which was 2.08 fold higher than the wild-type. Maximum reduction potential was found to be 0.237&plusmn;0.0118 which was 1.83 fold higher than wild-type. Mutagen concentration of 0.5 mM exhibited strong parietin activity. The extracts of EMS treated mutant variant revealed strong antimicrobial activity (22 mm for bacteria) as compared to the wild-type and MMS treated variant. It was concluded that by increasing mutagen exposure time, the parietin activity decreased in terms of its antimicrobial activity.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2019-JAPS-331"
}]