[{
  "type": "article-journal",
  "title": "INVESTIGATIONS ON ONOSMA HISPIDUM WALL ROOT EXTRACTS FOR IN-VITRO ANTIDIABETIC, PROLIFERATIVE AND CYTOTOXIC EFFECTS",
  "author": [
    {
      "family": "M.Asghar",
      "given": ""
    },
    {
      "family": "M.Islam",
      "given": ""
    },
    {
      "family": "H.Saeed",
      "given": ""
    },
    {
      "family": "F.Imtiaz",
      "given": ""
    },
    {
      "family": "B.Saleem",
      "given": ""
    },
    {
      "family": "Z.Saleem",
      "given": ""
    },
    {
      "family": "M.Iqtedar",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2018]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "28",
  "issue": "5",
  "page": "1339-1347",
  "DOI": "NA",
  "abstract": "Theaimofthisstudywastoinvestigatetheimpactofvariousextractsofrootsof Onosma hispidumforanti-diabetes, proliferativeandcytotoxiceffectsusing in-vitroassays.Phytochemicalscreeningofpowderandextractswasdoneby employing standard methods. Anticancer activity was assessed by using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazoliumbromide)andshorttermproliferationassayonHepG2cellline.Theantidiabeticpotentialwas determined byusing alpha-amylase inhibition assayand non-enzymatic glycosylation of haemoglobin assay. Data suggested that primary (CHO; 68.06 ± 0.19 and Proteins; 9.48 ± 0.27) and secondary metabolites (mg/g); max. Flavonoids (47.55 ± 0.13) in aqueous extract, max. Polyphenols (107.77 ± 0.57) in Pt. ether extract and max. glycosaponins(94.5±0.51)inchloroformextract,exhibitedvariationsamongvariousextractsobtainedfromrootsof Onosma hispidum.Petroleumetherandaqueousextractsexhibitedsignificantimpact(p<0.05)ontheproliferationof HepG2celllinesatdifferenttimepointscomparedtocontrol,whileincaseofMTTassay,nosignificantresults(p>0.05) wereobtainedafterovernightincubation.Improvedantidiabeticactivitywasexhibitedbypetroleumetherextract(70% inhibition)ascomparedtoAcarbosestandardusingalpha-amylaseinhibitionassay,withanIC of80µg/ml.Innon- 50 enzymatic glycosylation of haemoglobin assay, better potential was shown by chloroform extract with an IC of 50 35µg/ml,whencomparedtoastandard-Tocopherol.Thisstudyrevealedthattherootsof Onosma hispidumpossess nutritionalvalueandantidiabeticproperties,alongwithsignificantimpactoncell(HepG2)proliferation. and anti-mutagenic effects (Urquiaga and Leighton,",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2018-JAPS-513"
}]