[{
  "type": "article-journal",
  "title": "MOLECULAR CHARACTERIZATION AND PHYLOGENETIC ANALYSIS OF MYCOPLASMA SYNOVIAE ISOLATED FROM CHICKEN",
  "author": [
    {
      "family": "Rasool",
      "given": ""
    },
    {
      "family": "Anjum",
      "given": ""
    },
    {
      "family": "Rabbani",
      "given": ""
    },
    {
      "family": "Lateef",
      "given": ""
    },
    {
      "family": "Akhter",
      "given": ""
    },
    {
      "family": "Afroz",
      "given": ""
    },
    {
      "family": "Muhammad",
      "given": ""
    },
    {
      "family": "Nawaz",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2018]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "28",
  "issue": "2",
  "page": "491-497",
  "DOI": "NA",
  "abstract": "<p><em>Mycoplasma synoviae</em>&nbsp;is one of the paramount causes of economic losses for poultry industry. Present study was carried out with the aim to isolate, identify and characterize&nbsp;<em>M. synoviae</em>&nbsp;from suspected chicken. Tracheal and cloacal swabs samples (n=500), collected from suspected chicken, were cultured on Modified Frey, s medium for isolation of&nbsp;<em>Mycoplasma</em>. Preliminary identification of&nbsp;<em>Mycoplasma</em>&nbsp;was done by culturing and biochemical characterization. Partial 16 S r RNA was amplified and sequenced for confirmation and molecular identification of&nbsp;<em>M. synoviae</em>. For strain identification and phylogenetic analysis, partial amplification and sequence of vlhA gene was performed. Out of 500 samples,&nbsp;<em>Mycoplasma&nbsp;</em>was successfully isolated from 40 samples. Out of 40 mycoplasma isolates, 20 were identified as&nbsp;<em>M. synoviae&nbsp;</em>by specie specific 16S ribosomal RNA gene, partial amplification (~213 bp). The vlhA gene was also successfully partially amplified (~375 bp) and sequenced from five isolates. GenBank accession numbers of reported local isolates in this study are KJ130523-KJ130542.and KP316017-KP316021 respectively.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2018-JAPS-216"
}]