[{
  "type": "article-journal",
  "title": "EFFECT OF FUNGICIDES ON NITRATE UPTAKE AND ASSIMILATION IN ASPERGILLUSNIDULANS",
  "author": [
    {
      "family": "Amin",
      "given": ""
    },
    {
      "family": "Akhtar",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2017]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "27",
  "issue": "2",
  "page": "550-558",
  "DOI": "N/A",
  "abstract": "<p>In present study, effect of three fungicides, Iprobenfos, Fosetylaluminium and Azoxystrobin, on substrate specificity of NrtA and NrtB nitrate transporter proteins of&nbsp;<em>Aspergillus nidulans&nbsp;</em>has been studied<em>.&nbsp;</em>Azoxystrobin inhibited the growth of&nbsp;<em>A. nidulans</em>&nbsp;strains more strongly than Iprobenfos and Fosetyl aluminium. At0.25R of Iprobenfos, approximately 82%, 85%, 76% growth inhibition was observed by wild type,&nbsp;<em>nrtA1</em>&nbsp;and&nbsp;<em>nrtB110</em>respectively<em>.</em>&nbsp;Growth inhibition of wild type,&nbsp;<em>nrtA1</em>&nbsp;and&nbsp;<em>nrtB110</em>&nbsp;by recommended dose (R) of Fosetyl aluminium was 73%, 41% and 23%, respectively. 0.0075R of Azoxystrobin inhibited the growth of wild type,&nbsp;<em>nrtA1</em>&nbsp;and&nbsp;<em>nrtB110</em>by 51%, 68% and 71%, respectively. Effect of Iprobenfos, Fosety laluminium and Azoxystrobinwas also studied on nitrate uptake of the&nbsp;<em>A. nidulans</em>&nbsp;strains. At 0.25R of Iprobenfos, 94%, 54% and 100% nitrate uptake inhibition was observed by wild type,&nbsp;<em>nrtA1</em>&nbsp;and&nbsp;<em>nrtB110</em>&nbsp;respectively. Recommended dose of Fosety laluminiumcompletely stopped the nitrate uptake by all strains. At 0.01R of Azoxystrobin, complete cessation in nitrate uptakeby&nbsp;<em>nrtA1</em>&nbsp;and&nbsp;<em>nrtB110</em>was recorded however78% reduction in nitrate uptake was observedfor wild type strain.Increase in concentration of Iprobenfos(up to 0.25R), increasedthe nitrate reductase activity in wild type while decreased the enzymatic activity by<em>nrtA1</em>&nbsp;and&nbsp;<em>nrtB110</em>strains. Recommended dose of Fosetylaluminiumand 0.0075R of Azoxystrobinenhanced nitrate reductase activity in wild type and&nbsp;<em>nrtB110</em>, however reduced theenzymatic activity in&nbsp;<em>nrtA1</em>. Present study concludes that excessive use of fungicides is not only a threat for environment but also reduces the nitrate uptake and assimilation efficiency of eukaryotes.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2017-JAPS-69"
}]