[{
  "type": "article-journal",
  "title": "Morphological, physico-chemical and molecular investigations on Tuber bellonae from Basilicata-Italy",
  "author": [
    {
      "family": "Mang",
      "given": ""
    },
    {
      "family": "Zotta",
      "given": ""
    },
    {
      "family": "Camele",
      "given": ""
    },
    {
      "family": "Racioppi",
      "given": ""
    },
    {
      "family": "D’Auria",
      "given": ""
    },
    {
      "family": "Rana",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2017]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "27",
  "issue": "2",
  "page": "528-541",
  "DOI": "N/A",
  "abstract": "<p>Morphological, physico-chemical and molecular investigations were accomplished on ascomata of&nbsp;<em>Tuber bellonae</em>&nbsp;from Basilicata (southern Italy) identified on the basis of their macro- and microscopic features.&nbsp;<em>Tuber aestivum</em>&nbsp;and&nbsp;<em>T</em>.<em>&nbsp;mesentericum</em>&nbsp;were used as comparative truffle species. An accurate comparison of ascospore dimensions, in 4-spored asci, a morphological character previously used to distinguish the above described truffles, was also carried out. The volatile organic compounds (VOCs) from&nbsp;<em>T</em>.<em>&nbsp;bellonae</em>&nbsp;were investigated by solid phase microextraction technique coupled to gas chromatography and mass spectrometry (SPME-GC-MS). The following loci were used for molecular analysis: a) the beta-tubulin gene, b) the (GTG)5 microsatellite, c) the M13 minisatellite. The ascospore volume of&nbsp;<em>T</em>.<em>&nbsp;bellonae</em>&nbsp;in 4-spored asci resulted significantly bigger than those of&nbsp;<em>T</em>.<em>&nbsp;aestivum</em>&nbsp;and&nbsp;<em>T</em>.<em>&nbsp;mesentericum</em>. Twenty-three VOCs were detected. Out of these, only 1,4-dimethoxy-2-methylbenzene, never found in&nbsp;<em>T</em>.<em>&nbsp;aestivum&nbsp;</em>and<em>&nbsp;T</em>.<em>&nbsp;mesentericum</em>, could be considered the main VOC marker discriminating&nbsp;<em>T</em>.<em>&nbsp;bellonae</em>. High polymorphism level was revealed in&nbsp;<em>T. bellonae</em>&nbsp;by both microsatellite and minisatellite markers. Finally, preliminary results on partial &beta;-tubulin gene sequence of&nbsp;<em>T</em>.<em>&nbsp;bellonae</em>&nbsp;specimens suggest that&nbsp;<em>T</em>.<em>&nbsp;bellonae&nbsp;</em>is closely related to&nbsp;<em>T</em>.<em>&nbsp;aestivum</em>&nbsp;and&nbsp;<em>T</em>.<em>&nbsp;mesentericum,</em> probably holding an intermediate position between them.&nbsp;</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2017-JAPS-67"
}]