[{
  "type": "article-journal",
  "title": "CALLUS FORMATION FROM ISOLATED MICROSPORE CULTURE IN RADISH (RAPHANUS SATIVUS L.)",
  "author": [
    {
      "family": "Tuncer",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2017]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "27",
  "issue": "1",
  "page": "277-282",
  "DOI": "N/A",
  "abstract": "<p>In this study, the effects of applications of colchicine on microspore cultures were evaluated in 4 radish (<em>Raphanussativus</em> L.) varieties. For this purpose, 5 different concentrations of colchicine (0, 10, 25, 50, and 75 mg L-1) were applied under dark conditions at 4&deg;C to microspores that had been isolated in Gamborg&rsquo;s medium containing 13% (w/v)sucrose (B5-13). After the application of colchicine the microspores were kept for 1 day at 32.5&deg;C in1/2 x Nitsch and Nitschliquid media supplemented with13% (w/v)sucrose (1/2 NLN-13) at a density of 40.000 microspore mL-1and transferred for culturing under dark conditions at 25&deg;C. Colonies of callus were observed in Petri dishes 4&ndash;5 weeks after the microspore isolation. In this study, the 50 mg L-1 dose of colchicine was found to be more effective in terms of callus formation. The highest callus formation was achieved from the large round radish (cv. Burkır) (6.63 calli/Petri dish)and the standard small rooted radish (cv. Cherry Belle)(5.73 calli/Petri dish) varieties.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2017-JAPS-34"
}]