[{
  "type": "article-journal",
  "title": "DROUGHT-INDUCED TRANSCRIPTION OF RESISTANT AND SENSITIVE COMMON MILLET VARIETIES",
  "author": [
    {
      "family": "Wang",
      "given": ""
    },
    {
      "family": "Wang",
      "given": ""
    },
    {
      "family": "Liu",
      "given": ""
    },
    {
      "family": "Lian",
      "given": ""
    },
    {
      "family": "Chen",
      "given": ""
    },
    {
      "family": "Qiao",
      "given": ""
    },
    {
      "family": "McInerney",
      "given": ""
    },
    {
      "family": "Wang",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2017]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "27",
  "issue": "4",
  "page": "1303-1314",
  "DOI": "NA",
  "abstract": "<p>As a well-adapted plant to dry soil and short growing seasons, common millet holds great potential as a drought-resistant crop. Here, we performed a comparative RNA-Seq analysis of two common millet genotypes, Huangmizi (tolerant) and Zhenyuandamizi (sensitive) grown under stress conditions (20% Polyethylene Glycol 6000 with an average molecular weight of ～5,400-7,000 solution). Leaves of seedling plants were harvested from a control and also after 1 and 3 h stress treatment and pooled for RNA isolation. Using Illumina paired-end sequencing, approximately 46.5 million clean reads were generated. Following a<em>&nbsp;de novo</em> assembly, a total of 42,240 Unigenes were obtained. A total of 2,301 SSRs and 1,447,148 SNPs were also identified and 75% of Unigenes were annotated. A total of 21,556 and 8,304 Unigenes aligned to the GO and COG databases, respectively. The GO classification showed that the Unigenes were distributed into three categories with 74.30% having biological functions, 76.51% having molecular functions and 79.30% having cellular-level functions. By cross-referencing against KEGGs, 5,535 Unigenes were assigned to 63 metabolic pathways. Additionally, 701 DEGs including 187 up-regulated and 514 down-regulated genes were detected. Transcripts identified in this study will accelerate our understanding of the molecular mechanisms of drought tolerance in common millet.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2017-JAPS-165"
}]