[{
  "type": "article-journal",
  "title": "OPTIMIZING THE CONCENTRATION OF BIOTIN FOR L-GLUTAMIC ACID PRODUCTION BY A LOCALLY ISOLATED CORYNEFORM STRAIN",
  "author": [
    {
      "family": "Niaz1",
      "given": ""
    },
    {
      "family": "Rajoka",
      "given": ""
    },
    {
      "family": "Al-Ghanim",
      "given": ""
    },
    {
      "family": "Yousaf",
      "given": ""
    },
    {
      "family": "Mahboob",
      "given": ""
    },
    {
      "family": "Nadeem",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2017]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "27",
  "issue": "4",
  "page": "1217-1224",
  "DOI": "NA",
  "abstract": "<p>&nbsp;Various concentrations of biotin were tested in order to achieve fluent excretion of glutamic acid (glu) in a fermentation medium composed of a locally isolated&nbsp;<em>Corynebacterium glutamicum</em>&nbsp;strain. The behaviour of the strain was observed to vary with varying concentrations of biotin. Maximum values for specific growth rate (&mu;, h-1), cell mass yield (Yx/s, g/g), substrate consumption rate (QS, g/l h) and cell mass specific productivity (qX, g/g h) were 0.34, 0.24, 2.92 and 1.43, respectively, given 10 &mu;g biotin/100 ml under working conditions of a shake flask with temperature 30 &deg;C, in 100 g glucose/l medium (pH 7.0). The kinetic parameters calculated for glutamate production under the above conditions were 4.94 g/l h, 4.4 g/g cells, 0.66 g/g, and 1.5 g/g .h for QP, YP/X, YP/S, and qP, respectively, and were comparatively higher than the respective values reported for some&nbsp;<em>C. glutamicum</em>&nbsp;strains growing on glucose in batch culture studies. Conversely, at biotin concentrations of 1.0, 2.5, 5.0, 15 &mu;g/ ml, significantly less improvement in&nbsp;<em>glu</em> production was noticed, but at 10 &mu;g/ml a nearly 2.2-fold increase in QP was observed, though the only statistically significant difference was in the QS value.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2017-JAPS-154"
}]